Tissue culture media is a buffered nutrient solution used in laboratories to grow mammalian tissue culture cells for experiments. When cell biologists grow mammalian tissue culture cells a major issue is media acidification caused by the cells excreting metabolic byproducts. Media makers must be careful not to include too much solute in their media, as it can kill the cells through osmotic pressure imbalances, so getting the balance right is important. Based on this, if you were designing tissue culture media what ratio of acid to base would you think is best for the buffer component of your media? O90% acid: 10% base 25% acid: 75% base 75% acid: 25% base base 10% acid: 90% base O 50% acid: 50%
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- Identify (state whether each item is an example of diffusion, osmosis, filtration, or active transport) 1. Movement of water from an area of lower impermeant solute concentration to an area of higher impermeant solute concentration. 2. Dye particles spread evenly through water. 3. Starch particles pass through a paper membrane. 4. A cell uses energy to "pump" sugar molecules from its external environment. 5. Water in cell moves out into a concentrated salt solution bathing the cell.determine if osmosis or simple diffusion A crystal of blue copper sulfate is placed in a test tube of water. The next day, the solid is gone, but the water is evenly colored. A fresh potato is peeled, weighed, and soaked in a strong salt solution. The next day, it is discovered that the potato has lost a significant amount of mass. A perfume bottle is opened, and soon the odor can be sensed in all parts of the room. Garden grass wilts after being exposed to dry chemical fertilizer. Plant seeds soaked in water swell and become several times as large as before soaking. A sugar cube is dropped into a cup of hot water, and without being stirred, all of the liquid becomes sweet tasting.Various chemical methods can be used to permeabilize or lyse cells including the use of enzymes. List three important factors to consider when developing a lysis method using enzymes. Estimate the osmotic pressure drop across the membrane of an animal cell in a 0.10 M NaCl solution, assuming that the internal total solute concentration is 0.36 osM and temperature is 30.0°C. Do you think that this pressure drop would lyse cells? Why or why not?