Refer to the codon diagram on. Which of the following is a codon that will terminate translation? * Phe Leu Glu (E) AGU C Cys (C) GU (3) Trp (W) G А С C UG Arg (R) Leu Ser (S) Lys (K) C. Pro Asn (N) His Thr (H) Gin (Q) Arg (R) lle (1) Siar 是3 Met (M)
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- Refer to the codon diagram on. Which of the following is a codon that will terminate translation?* ates es Ders rences anline Gly (G) Leu (F) Ser (S) Ty 3. Asp (E) (D) C la Ala (A) GU Cys (C) eriods 1 and 2 G ds P1, Highschool pol MP3, P4 Trp (W) AC Arg (R) Leu (L) Ser (S) Lys (K) C. Pro Asn (N) (P) His The (H) Thu Fri Gin (0) INTLGiven the following codons and their corresponding amino acids: UUU-Phenylalanine GAA- Glutamate CAA- Glutamine AAU- Asparagine AAC- Asn AAA- Lysine UCU- Serine GGA-Glycine ACC-Threonine AUG- Met/ START codon CCU- Proline GUU- Valine UAU-Tyrosine UAA- STOP AGG- Arginine AUU- Isoleucine CAU- Histidine GCU- Alanine UGU-Cysteir GAU-Asparti CUA-Leucine UGG-Tryptol CGU-Arginin Box 1: Show the mRNA sequence which codes for the short peptide, lys-ala-phe- leu. Include what should come before and after this short message. Don't leave any spaces between the letters. Box 2: Show the tRNA anticodon sequence that would line up with the mRNA strand from Box 1. Don't leave any spaces between the letters. Box 3 & 4: Show the DNA base sequence that would be found in the DNA double helix which carries the gene for this peptide. Give the coding strand sequence in Box 3 and template strand sequence in Box 4. Don't leave any spaces between the letters. Box 5: What if there was a frameshift at leucine…Val (V) Ala (A) Arg (R) Ser (S) Lys (K) Start Stop Using the codon wheel below, identify which of the following mutations of the DNA sequence TTT TAC ACT would potentially have the least effect on the protein containing the sequence? Asp (D) G A JOOGA C Asn (N) اندان A داد Glu (E) 9/2/3. 20402 CAGUCAGUCAGUC GU ט|כ Thr C U G G A Gly A A Phe (F) C/U/G ●Met (M) Leu (L) GU AC C CUGA GACUGA ACUGACUG A Arg (R) G כט Ser (S) C U с Gin (Q) A G U C A G બનીનો His (H) Tyr (Y) U G C Cys (C) Pro (P) Trp (W) Leu (L) A) TTA TAC ACT B) TTT TTC TCT C) TTT TAC TGA D) TAA AAC AGA E) They would all have the same effect.
- Using the Genetic Code Table in Figure 1.3, what is the proper sequence of amino acids in the polypeptide chain based from the given codons of MRNA (3' AAU GCC AGU GGU 5')? * U UUU Phe UCU) UC UCA UCG UAU] UAC Tyr UAA Stop UGA Stop A UAG Stop UGG Trp G UGU U UUC) UGC Cys Ser UUA Leu UUGJ CU) CCU CAU1 CGU His CUC CAC CAA CGC CC Leu CCA Pro Arg CUA CGA A Gin CUG) CG CAG CGG G AUU) ACU) AGU AAU1 Asn AAC, Thr AAA1 U AGC }Ser č A G AUC lle ACC A AUA J ACA AGA AUG Met ACG, AAG}Lys AGG Arg GGU GUU) GUC Val GCU) GCC GCA GCG J GAU1 Asp GACI Ala GGC GGA GGG ) G GUA Gly GAA1 GAG) A Glu GUG) G Figure 1.3 Alanine, Serine, Glycine, Asparagine Serine, Glycine, Asparagine, Alanine Glycine, Asparagine, Alanine, Serine Asparagine, Alanine, Serine, Glycine5'-ATGCTGCGTGCATGGGATATAGGTAGCACACGTCC-3' 3'-TACGACGCACGTACCC TATATCC ATCGTGTGCAGG-5' (a) Assuming that transcription starts with the first C in the template strand, and continues to the end, what would be the sequence of the MRNA derived from this fragment? (b) Find the initiation and stop codons in this MRNA. (c) Would there be an effect on translation of changing the fourth T in the template strand to a C? If so, what effect?From this overall anticodon sequence in tRNA, 3'-CAUCGGAAUAGAUCGCUAGUGGCAGGCAUAAUGAUCACCGGUCUGAGAAAAGUGGUACAUAUCAAC-5' What is the amino acid sequence that will be coded for using ONE-letter amino acid code starting from N-terminus to C-terminus and using THREE-letter amino acid code starting from N-terminus to C-terminus
- According to wobble rules, what codons should be recognized by the follow- ing anticodons? What amino acid residues do these correspond to? (а) 5' —1CC—3' (b) 5'-GCU-3'From this overall anticodon sequence in tRNA, 3'-CAUCGGAAUAGAUCGCUAGUGGCAGGCAUAAUGAUCACCGGUCUGAGAAAAGUGGUACAUAUCAAC-5' Using ONE-letter amino acid code starting from N-terminus to C-terminus, what is the amino acid sequence that will be coded for?. The genetic code is thought to have evolved to maximize genetic stability by minimizing the effect on protein function of most substitution muta- tions (single-base changes). We will use the six arginine codons to test this idea. Consider all of the substitutions that could affect all of the six arginine codons. (a) How many total mutations are possible? (b) How many of these mutations are "silent," in the sense that the mutant codon is changed to another Arg codon? (c) How many of these mutations are conservative, in the sense that an Arg codon is changed to a functionally similar Lys codon?
- a) b) Shown below is a DNA sequence that encodes for a section of a protein. Please write the amino acid sequence using the three letter codes for this section. 5' ATG ACT CTC TCC TGG GGC ATC CGA TAA 3' What would the second codon be changed to if it was both a silent mutation and a transition mutation? Please write an anticodon in 5' to 3' direction that would recognize both the original second codon and the mutated second codon.Write the sequence of the mRNA molecule synthesized from a DNA template strand having the sequence 5'-ATTACAGGCGGT-3' 5'- UAAUGUCCGCCA Incorrect Write the amino acid sequence encoded by the mRNA base sequence 5'-GAGUUAGUUUGUAAGUGC-3' Assume the reading frame starts at the 5' end. Refer to the codon table . Amino acid sequence: Glu-Leu-Val-Cys-Lys-Cys What is the sequence of the polypeptide formed on addition of poly(UUAC) to a cell-free protein-synthesizing system that does not require a start codon? Enter an amino acid sequence of four amino acids using the three-letter abbreviations. Polypeptide sequence: Poly( -3' Glu-Leu-Val-Cys-Lys-CysACUGACUGA Use the codon chart to determine the following RNA strand in amino acids (Remember to write it the same way the strand is): AUG-AGU-GGG-UGU Gly Phe Leu (F) (L) Glu Ser Asp (E) (D) (S) AGU C Tyr (Y) Ala AGU (A) A Val Cys (C) G U (V) C G A 34 Trp (W)3 G G Arg (R) U AG A C C Leu Ser (S) U (L) A C G. A Lys (K) CUG A Pro Asn (N) CUGACU G (P) His Thr Gln (H) (Q) Arg (R) Start Stop Your answer Met (M)O