Rate in U [S] in mM No Inhibitor 0.5 1.0 2.0 4.0 8.0 16.0 32.0 64.0 0.22 0.41 0.73 1.12 1.57 2.01 2.3 2.5 3.0 mM Inhibitor Copy and paste the above data into Excel. Create an Eadie-Hofstee plot of the give dataset. 0.08 0.15 0.29 0.52 0.85 1.3 1.78 2.16 Calculate the Vmax, and Vmax/ Km parameters for both the uninhibited and inhibited trials (Inhibited parameters are marked with an apostrophe (')). Finally, determine the mechanism of inhibition. Note: give all parameters to two decimal places, and do not include the units. For th method of inhibition, state competitive, uncompetitive, or mixed as your answer. Vmax 1. Vmax= U-mM-1. Mechanism of inhibition = inhibition N U. Vmax/Km U. Vmax/Km= A U.mM

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter9: Parenteral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 7.7P
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Rate in U
[S] in mM No Inhibitor
0.5
1.0
2.0
4.0
8.0
16.0
32.0
64.0
0.22
0.41
0.73
1.12
1.57
2.01
2.3
2.5
3.0 mM
Inhibitor
Copy and paste the above data into Excel. Create an Eadie-Hofstee plot of the given
dataset.
0.08
0.15
0.29
0.52
0.85
1.3
1.78
2.16
Calculate the Vmax, and Vmax/ Km parameters for both the uninhibited and inhibited
trials (Inhibited parameters are marked with an apostrophe (')).
Finally, determine the mechanism of inhibition.
Note: give all parameters to two decimal places, and do not include the units. For the
method of inhibition, state competitive, uncompetitive, or mixed as your answer.
U. Vmax/Km-
A U. Vmax/Km=
Vmax
1. Vmax=
U-mM-1. Mechanism of inhibition =
inhibition
A/
A/
A/
U.mM
A
Transcribed Image Text:Rate in U [S] in mM No Inhibitor 0.5 1.0 2.0 4.0 8.0 16.0 32.0 64.0 0.22 0.41 0.73 1.12 1.57 2.01 2.3 2.5 3.0 mM Inhibitor Copy and paste the above data into Excel. Create an Eadie-Hofstee plot of the given dataset. 0.08 0.15 0.29 0.52 0.85 1.3 1.78 2.16 Calculate the Vmax, and Vmax/ Km parameters for both the uninhibited and inhibited trials (Inhibited parameters are marked with an apostrophe (')). Finally, determine the mechanism of inhibition. Note: give all parameters to two decimal places, and do not include the units. For the method of inhibition, state competitive, uncompetitive, or mixed as your answer. U. Vmax/Km- A U. Vmax/Km= Vmax 1. Vmax= U-mM-1. Mechanism of inhibition = inhibition A/ A/ A/ U.mM A
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