The data in the table are used to create a calibration curve for the determination of RNA from ts absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m = b= RNA (µg) 0.00 10.36 20.72 31.08 41.44 Absorbance at 260 nm 0.268 0.497 0.795 1.131 1.373 Hg Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.699.
The data in the table are used to create a calibration curve for the determination of RNA from ts absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m = b= RNA (µg) 0.00 10.36 20.72 31.08 41.44 Absorbance at 260 nm 0.268 0.497 0.795 1.131 1.373 Hg Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.699.
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter5: Biomedical Electronics: Digital
Section: Chapter Questions
Problem 4P
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Transcribed Image Text:The data in the table are used to create a calibration curve for the determination of RNA from
ts absorbance at 260 nm.
Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the
best straight line fit to the calibration curve. Do not subtract the blank reading when creating
the calibration curve.
m=
b =
RNA
(µg)
0.00
10.36
20.72
31.08
41.44
Absorbance at 260
nm
0.268
0.497
0.795
1.131
1.373
µg
Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of
0.699.
-1
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