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QUESTION 56 PDGF 100 740 751 L.. PI3K 50 GAP O GAP 771 protein P-site PTP O 4 1009 O PTP PI3K 740, 751+ 1021 GAP PTP 771 1009 PLC-y PLC PLCY 1021 When activated by ligand binding, the PDGF (platelet-derived growth factor) receptor becomes phosphorylated on 5 tyrosine residues (left figure). These phosphorylated tyrosines serve as binding sites for proteins that contain SH2 domains (SH2 domains bind phosphorylated Y). These proteins include phospholipase C-gamma (PLC-gamma), a phosphotyrosine phosphatase (PTP), a Ras GTPase- Activating Protein (GAP), and a phosphotidylinositol 3-Kinase (PI3K). PDGF stimulates several changes in the target cell, one of which is DNA synthesis. To determine which effectors of the PDGF receptor is/are responsible for stimulating DNA synthesis, you construct several mutant forms of the receptor that retain individual or combinations of the phosphorylation sites. You express these in cells and monitor DNA synthesis. The results are shown in the right figure. Which effector suppresses DNA synthesis? O a. PTP O b. PI3K O c. GAP O d. PLC-gamma

QUESTION 56 PDGF 100 740 751 L.. PI3K 50 GAP O GAP 771 protein P-site PTP O 4 1009 O PTP PI3K 740, 751+ 1021 GAP PTP 771 1009 PLC-y PLC PLCY 1021 When activated by ligand binding, the PDGF (platelet-derived growth factor) receptor becomes phosphorylated on 5 tyrosine residues (left figure). These phosphorylated tyrosines serve as binding sites for proteins that contain SH2 domains (SH2 domains bind phosphorylated Y). These proteins include phospholipase C-gamma (PLC-gamma), a phosphotyrosine phosphatase (PTP), a Ras GTPase- Activating Protein (GAP), and a phosphotidylinositol 3-Kinase (PI3K). PDGF stimulates several changes in the target cell, one of which is DNA synthesis. To determine which effectors of the PDGF receptor is/are responsible for stimulating DNA synthesis, you construct several mutant forms of the receptor that retain individual or combinations of the phosphorylation sites. You express these in cells and monitor DNA synthesis. The results are shown in the right figure. Which effector suppresses DNA synthesis? O a. PTP O b. PI3K O c. GAP O d. PLC-gamma

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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QUESTION 56 PDGF HILL 100 PI3K PI3K 751 50 GAP O O GAP 771 protein Psite 1 2 PTP O 1009 O PTP PI3K 740, 751 GAP 771 1021 PTP 1009 PLC PLC PLC-y 1021 When activated by ligand binding, the PDGF (platelet-derived growth factor) receptor becomes phosphorylated on 5 tyrosine residues (left figure). These phosphorylated tyrosines serve as binding sites for proteins that contain SH2 domains (SH2 domains bind phosphorylated Y). These proteins include phospholipase C-gamma (PLC-gamma), a phosphotyrosine phosphatase (PTP), a Ras GTPase- Activating Protein (GAP), and a phosphotidylinositol 3-Kinase (PI3K). PDGF stimulates several changes in the target cell, one of which is DNA synthesis. To determine which effectors of the PDGF receptor is/are responsible for stimulating DNA synthesis, you construct several mutant forms of the receptor that retain individual or combinations of the phosphorylation sites. You express these in cells and monitor DNA synthesis. The results are shown the right figure. Which effector suppresses DNA synthesis? O a. PTP O b. PI3K O c. GAP O d. PLC-gamma
Transcribed Image Text:QUESTION 56 PDGF HILL 100 PI3K PI3K 751 50 GAP O O GAP 771 protein Psite 1 2 PTP O 1009 O PTP PI3K 740, 751 GAP 771 1021 PTP 1009 PLC PLC PLC-y 1021 When activated by ligand binding, the PDGF (platelet-derived growth factor) receptor becomes phosphorylated on 5 tyrosine residues (left figure). These phosphorylated tyrosines serve as binding sites for proteins that contain SH2 domains (SH2 domains bind phosphorylated Y). These proteins include phospholipase C-gamma (PLC-gamma), a phosphotyrosine phosphatase (PTP), a Ras GTPase- Activating Protein (GAP), and a phosphotidylinositol 3-Kinase (PI3K). PDGF stimulates several changes in the target cell, one of which is DNA synthesis. To determine which effectors of the PDGF receptor is/are responsible for stimulating DNA synthesis, you construct several mutant forms of the receptor that retain individual or combinations of the phosphorylation sites. You express these in cells and monitor DNA synthesis. The results are shown the right figure. Which effector suppresses DNA synthesis? O a. PTP O b. PI3K O c. GAP O d. PLC-gamma
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