Polymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange column. Th total mass of beads in the column is 8.47 kg. On average, each bead weighs 0.0023 g and has an average of 18. 10° positively charged amine groups that can adsorb a negatively charged protein that passes through the column. A solution containing 2.07 mg/L of a protein is maintained at pH 6.3 and is passed through the ion exchange column at 0.215 L/min. The protein has a molecular weight of 154,000. The pks of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 5.6. A. How long can the column be operated before reaching 80% capacity (i.e., 80% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). B. After reaching 80% capacity, explain what you would do to release the protein attached to the beads? (to ge the isolated product and regenerate the ionic exchange resin). C. Would changing the pH to 10.0 alter the capacity of the DEAE beads to bind the protein? Explain. D. Would changing the pH to 4.0 alter the capacity of the DEAE beads to bind the protein? Explain. Note: Avogadro's number is 6.022 10. Don't look for formulas for this problem- we haven't gone over any
Polymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange column. Th total mass of beads in the column is 8.47 kg. On average, each bead weighs 0.0023 g and has an average of 18. 10° positively charged amine groups that can adsorb a negatively charged protein that passes through the column. A solution containing 2.07 mg/L of a protein is maintained at pH 6.3 and is passed through the ion exchange column at 0.215 L/min. The protein has a molecular weight of 154,000. The pks of the amino groups on DEAE cellulose is 7.1, and the pl of the protein is 5.6. A. How long can the column be operated before reaching 80% capacity (i.e., 80% of the amino groups on DEAE are bound to the protein through an ionic bond)? You may assume that one protein attaches to one + charge on the beads (although it's possible that proteins attach to more than one + charge). B. After reaching 80% capacity, explain what you would do to release the protein attached to the beads? (to ge the isolated product and regenerate the ionic exchange resin). C. Would changing the pH to 10.0 alter the capacity of the DEAE beads to bind the protein? Explain. D. Would changing the pH to 4.0 alter the capacity of the DEAE beads to bind the protein? Explain. Note: Avogadro's number is 6.022 10. Don't look for formulas for this problem- we haven't gone over any
Chapter11: Health Of Aquatic Animals
Section: Chapter Questions
Problem 3KA
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Transcribed Image Text:Polymer beads (resin) made of DEAE (diethylaminoethyl) cellulose are packed in an ion exchange column. The
total mass of beads in the column is 8.47 kg. On average, each bead weighs 0.0023 g and has an average of 18.4
* 10° positively charged amine groups that can adsorba negatively charged protein that passes through the
column. A solution containing 2.07 mg/L of a protein is maintained at pH 6.3 and is passed through the ion
exchange column at 0.215 L/min. The protein has a molecular weight of 154,000. The pk, of the amino groups
on DEAE cellulose is 7.1, and the pl of the protein is 5.6.
2.
A. How long can the column be operated before reaching 80% capacity (i.e., 80% of the amino groups on DEAE
are bound to the protein through an ionic bond)? You may assume that one protein attaches to one +
charge on the beads (although it's possible that proteins attach to more than one + charge).
B. After reaching 80% capacity, explain what you would do to release the protein attached to the beads? (to get
the isolated product and regenerate the lonic exchange resin).
C. Would changing the pH to 10.0 alter the capacity of the DEAE beads to bind the protein? Explain.
D. Would changing the pH to 4.0 alter the capacity of the DEAE beads to bind the protein? Explain.
Note: Avogadro's number is 6.022* 10". Don't look for formulas for this problem- we haven't gone over any
formulas for this. You can work through this problem by understanding how ion exchange works and using logic
and dimensional analysis; the units on variables will be helpful in getting to the answer.
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