obtain micro centrifuge tubes that contain each enzyme stock solution. digest the lambda DNA with a specific restriction enzyme. incubate the mixture of DNA and enzyme run the restriction digested DNA with loading dye in the agarose gel analyze the DNA fragments A. 4th event B. 2nd event C. 1st event D. 3rd event E. 5th event

obtain micro centrifuge tubes that contain each enzyme stock solution. digest the lambda DNA with a specific restriction enzyme. incubate the mixture of DNA and enzyme run the restriction digested DNA with loading dye in the agarose gel analyze the DNA fragments A. 4th event B. 2nd event C. 1st event D. 3rd event E. 5th event

Biology: The Unity and Diversity of Life (MindTap Course List)

15th Edition

ISBN:9781337408332

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter15: Studying And Manipulating Genomes

Section: Chapter Questions

Problem 16SQ: Match the method with the appropriate enzyme. _____ PCR a. Taq polymerase _____ cutting DNA b. DNA...

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Select all the following statements that are correct regarding agarose gel electrophoresis and restriction digests. Group of answer choices A higher percentage of agarose is used to distinguish between smaller pieces of DNA DNA fragments run from the positive electrode (where they are loaded) to the negative electrode Buffer is poured into the electrophoresis chamber AFTER the DNA has been loaded into the lanes. Larger DNA fragments migrate more slowly than smaller DNA fragments Loading dye is added AFTER restriction digests have been completed.
B. Restriction Mapping. Single and double digestion of plasmid pMCS326 were performed using the restriction enzymes Alulll and EcoRV. DNA fragments are shown in an electrophoretogram below. Construct a restriction map of plasmid pMCS326 for enzymes Alulll and EcoRV. 20 kb 11 kb 8 kb 6 kb kb 3 Alulll + Alull EcoRV ECORV | || Restriction Map:
Part A. If student counts 63 colonies on their 10^-6 dilution LB plate. What was the original concentration of their cells if they plated 100ul? Part B.If we used pGFPuv as the template for PCR positive control. This is because: a. it contains the GFP gene so it should show a product. b. It contains DNA fragments that were added to the ligation reaction. c. It is the desired plasmid we wanted to make. d. So we have a band to compare our unknown plasid to allowing us to check if the unknown is the right size.
Examine the pGLO plasmid DNA solution with the UV lamp. Note your observations. Using a micropipettor, withdraw 10 ul of plasmid and mix it into the cell suspension of the +PGLO tube by pipetting up and down three times. Close the tube and return it to the rack on ice. Also close the -PGLO tube. Do not add plasmid DNA to the -pGLO tube. Why not?
Some Steps Involved in Creating Recombinant DNA 1. Plasmid DNA is cut at the restriction site.2. Foreign DNA is cut at the restriction site.3. Plasmid DNA is opened and sticky ends are formed.4. Foreign DNA restriction fragments are isolated5. Target sequence in the plasmid is recognized by the restriction endonuclease.6. Target sequences in the foreign DNA are recognized by the restriction endonuclease.7. Foreign DNA restriction fragment is inserted into the plasmid at the restriction site.8. DNA ligase splices the foreign restriction fragment and the plasmid together. Identify the correct sequence of steps involved in cutting and reassembling DNA molecules to make recombinant DNA. Start with preparation of the plasmid DNA. Select one: a. 6, 2, 4, 7, 8, 5, 1, 3 b. 5, 1, 3, 6, 2, 4, 8, 7 c. 5, 1, 3, 6, 2, 4, 7, 8 d. 6, 2, 4, 5, 1, 3, 8, 7
catalyzes the cleavage of DNA at a specific ii fragments. The statement given above is completed nucleotide sequence. splices DNA into by the information in row Row i ii A. DNA polymerase DNA ligase B. restriction DNA ligase endonuclease DNA Ligase restriction endonuclease restriction endonuclease С. D. restriction endonuclease A OB C D
DNA scissors used in genetic engineering applications are called Endo nucleases Restriction enzymes Exo nucleases O DNases
For the analysis of plasmid DNA samples, ________ that recognize, bind to and cleave DNA at specific nucleotide sequences are used. Question options: primase restriction enzyme. DNA polymerase I. reverse transcriptase.
The other options are: a. RNA cannot be digested by restriction enzymes b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion. c. RNA is single stranded and DNA is double stranded
digest suspect B's DNA using restriction enzyme 1. Restriction enzyme 1 cleaves the recognition site 5' -AA/TT -3' . And count the fragment sizes from the digestion of the suspect.
Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…
Based on the image below, answer the following questions: Restriction enzymes PstI and ScaI were employed and the gel image after restriction digest is shown below. The name of the plasmid is Blank 1. The gel lane on the Blank 2 was digested using PstI enzyme. (left or right) The gel lane on the Blank 3 was digested using PstI and ScaI enzymes. (left or right)