Nutrient supplementation in medium Presence Medium Ade Arg Cys His Leu Pro of colonies 3 C 4 z zuzu z + + + + + I + + + + I + + + +1 + + + + I + + + +I + + + + I + + + + + 1.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
An ade+ arg+ cys+ his+ leu+ pro+ bacterial strain is known
to be lysogenic for a newly discovered phage, but the site
of the prophage is not known. The bacterial map is
leu
cys
arg
his
ade
pro
The lysogenic strain is used as a source of the phage, and
the phages are added to a bacterial strain of genotype
ade
- arg
- cys
- his- leu- pro-. After a short incubation,
samples of these bacteria are plated on six different
media, with the supplementations indicated in the
following table. The table also shows whether colonies
were observed on the various media.
Presence
Medium Ade Arg Cys His Leu Pro of colonies
1 - + + + + + N
2 + - + + + + N
3 + + - + + + C
4 + + + - + + N
5 + + + + - + C
6 + + + + + - N
Nutrient supplementation in medium
(In this table, a plus sign indicates the presence of a
nutrient supplement, a minus sign indicates that a
supplement is not present, N indicates no colonies, and C
indicates colonies present.)
a. What genetic process is at work here?
b. What is the approximate locus of the prophage?
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