I need help writting a biological "title" for this lab report. I performed 5 labs which are PCR purification, Rapid ligation, and Transformation, Blue/White screening and observation of pGreen transformation, Plasmid Extraction and Restriction Enzyme Digestion, Soil DNA extraction and gel electrophoresis, and Bioinformatics Analysis of 16s rRNA genes.

Note; I attached one page of my abstract and one page of the introduction
Transcribed Image Text:Abstract: We started by performing PCR purification, rapid ligation, and transformation experiments. The reason we performed this experiment is to remove the active thermophilic DNA polymerase present in the PCR mixture, catalyze the formation of phosphodiester presence of ATP between double-stranded DNA with 3'hydroxyl and 5' phosphate termini, and to transform the Escherichia coli bacteria when expose it to extracellular plasmid DNA that contains the pGreen gene and the gene for ampicillin resistance. In this experiment, we got 48.1 for the DNA concentration and 1.82 for the purity. Next, we performed white/blue screening and observed a pGreen transformation. The purpose of the experiment is to perform a white/ blue screening plate from the previous experiment along with preparing and viewing slides of transformed E. coli with the pGreen plasmid under the fluorescence microscope. But unfortunately the culture/ plates were contaminated from the previous lab so we just used the plates that had the control. Then, we did a plasmid extraction and restriction enzyme digestion lab and the purpose was to cut the plasmid into specific sized pieces and analyze the resulting fragments by gel electrophoresis. For the DNA concentration we got 46.7 and the purity was 2.05. After that we performed a soil DNA extraction and gel electrophoresis. The main purpose for DNA extraction in general was to study the genetic causes of disease and for the development of diagnostics and drugs, sequencing genomes, and detecting bacteria and viruses. The DNA concentration was 22.3 ng/uL and the purity was 1.92. Finally, we performed a Bioinformatics analysis of 16s TRNA genes to get results for BLAST identify a strain using the EzTaxon
Transcribed Image Text:Introduction: Purification of DNA from a PCR reaction is typically necessary for downstream use, and facilitates the removal of enzymes, nucleotides, primers and buffer components. ligation is performed by the T4 DNA ligase enzyme. The DNA ligase catalyzes the formation of covalent phosphodiester linkages, which permanently join the nucleotides together. After ligation, the insert DNA is physically attached to the backbone and the complete plasmid can be transformed into bacterial cells for propagation. There are three main steps of gene cloning: 1) Isolation and fragmentation of source DNA, 2) Inserting DNA fragments into cloning vectors, and 3) Introduction of cloned DNA into host organisms. Also, PUC19 is used for cloning vectors that convey the ampicillin resistance. T-vector is useful for cloning PCR products because of its T3' overhang. Next, we started by performing the Blue/white screening and observation of pGreen transformation. Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of ß-galactosidase, an enzyme occurring in E. coli, which cleaves lactose into glucose and galactose. Transformation is an introduction of a plasmid vector with foreign DNA inserted into competent E. coli Any colony containing the plasmid (and therefore the functioning B-galactosidase gene) will turn blue, a result of the B-galactosidase activity. Those colonies containing plasmids with an insert can be differentiated from those without an insert by the color of the colony (white versus blue). The insert disrupted the B-galactosidase gene, and therefore these colonies remain white. Colonies that did not pick up

Question

I need help writting a biological &#34;title&#34; for this lab report. I performed 5 labs which are PCR purification, Rapid ligation, and Transformation, Blue/White screening and observation of pGreen transformation, Plasmid Extraction and Restriction Enzyme Digestion, Soil DNA extraction and gel electrophoresis, and Bioinformatics Analysis of 16s rRNA genes. Note; I attached one page of my abstract and one page of the introduction Abstract:We started by performing PCR purification, rapid ligation, and transformationexperiments. The reason we performed this experiment is to remove the active thermophilicDNA polymerase present in the PCR mixture, catalyze the formation of phosphodiester presenceof ATP between double-stranded DNA with 3'hydroxyl and 5' phosphate termini, and totransform the Escherichia coli bacteria when expose it to extracellular plasmid DNA thatcontains the pGreen gene and the gene for ampicillin resistance. In this experiment, we got 48.1for the DNA concentration and 1.82 for the purity. Next, we performed white/blue screening andobserved a pGreen transformation. The purpose of the experiment is to perform a white/ bluescreening plate from the previous experiment along with preparing and viewing slides oftransformed E. coli with the pGreen plasmid under the fluorescence microscope. Butunfortunately the culture/ plates were contaminated from the previous lab so we just used theplates that had the control. Then, we did a plasmid extraction and restriction enzyme digestionlab and the purpose was to cut the plasmid into specific sized pieces and analyze the resultingfragments by gel electrophoresis. For the DNA concentration we got 46.7 and the purity was2.05. After that we performed a soil DNA extraction and gel electrophoresis. The main purposefor DNA extraction in general was to study the genetic causes of disease and for the developmentof diagnostics and drugs, sequencing genomes, and detecting bacteria and viruses. The DNAconcentration was 22.3 ng/uL and the purity was 1.92. Finally, we performed a Bioinformaticsanalysis of 16s TRNA genes to get results for BLAST identify a strain using the EzTaxon Introduction:Purification of DNA from a PCR reaction is typically necessary for downstream use, andfacilitates the removal of enzymes, nucleotides, primers and buffer components. ligation isperformed by the T4 DNA ligase enzyme. The DNA ligase catalyzes the formation of covalentphosphodiester linkages, which permanently join the nucleotides together. After ligation, theinsert DNA is physically attached to the backbone and the complete plasmid can be transformedinto bacterial cells for propagation. There are three main steps of gene cloning: 1) Isolation andfragmentation of source DNA, 2) Inserting DNA fragments into cloning vectors, and 3)Introduction of cloned DNA into host organisms. Also, PUC19 is used for cloning vectors thatconvey the ampicillin resistance. T-vector is useful for cloning PCR products because of its T3'overhang. Next, we started by performing the Blue/white screening and observation of pGreentransformation. Blue-white screening is a rapid and efficient technique for the identification ofrecombinant bacteria. It relies on the activity of ß-galactosidase, an enzyme occurring in E. coli,which cleaves lactose into glucose and galactose. Transformation is an introduction of a plasmidvector with foreign DNA inserted into competent E. coli Any colony containing the plasmid(and therefore the functioning B-galactosidase gene) will turn blue, a result of the B-galactosidaseactivity. Those colonies containing plasmids with an insert can be differentiated from thosewithout an insert by the color of the colony (white versus blue). The insert disrupted theB-galactosidase gene, and therefore these colonies remain white. Colonies that did not pick up

Accepted Answer

" bioinformatic analysis and the recombination techniques ".