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Making Mammalian Cell Growth Medium 1. The cells that you are working with require the following constituents in the growth medium: 10 mM glucose 4 mM glutamine 1 mM pyruvate 8.3 g/L DMEM 3.7 g/L NaHCO3 10% FBS 2. Take time to calculate the appropriate amount of each of the above items to prepare 100 ml of growth medium using the prepared liquid stock reagents. ***Check with your laboratory instructor before proceeding.

Making Mammalian Cell Growth Medium 1. The cells that you are working with require the following constituents in the growth medium: 10 mM glucose 4 mM glutamine 1 mM pyruvate 8.3 g/L DMEM 3.7 g/L NaHCO3 10% FBS 2. Take time to calculate the appropriate amount of each of the above items to prepare 100 ml of growth medium using the prepared liquid stock reagents. ***Check with your laboratory instructor before proceeding.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Materials Provided per Group of 4 students One 10 cm dish of 80% Confluent A549 cells One sterile 10 cm cell culture plate 5 ml and 10 ml sterile serological pipettes Sterile Pasteur pipettes Sterile ddH2O 20 ml sterile FBS 20 ml sterile 100 mM glucose 20 ml sterile 200 mM glutamine 20 ml sterile 100 mM pyruvate 20 ml sterile 83 g/L DMEM 20 ml sterile 37 g/L NaHCO3 Sterile 250 ml glass bottle 100 ml sterile 1X PBS 20 ml sterile TrypLE One sterile 15 ml conical tube
Transcribed Image Text:Materials Provided per Group of 4 students One 10 cm dish of 80% Confluent A549 cells One sterile 10 cm cell culture plate 5 ml and 10 ml sterile serological pipettes Sterile Pasteur pipettes Sterile ddH2O 20 ml sterile FBS 20 ml sterile 100 mM glucose 20 ml sterile 200 mM glutamine 20 ml sterile 100 mM pyruvate 20 ml sterile 83 g/L DMEM 20 ml sterile 37 g/L NaHCO3 Sterile 250 ml glass bottle 100 ml sterile 1X PBS 20 ml sterile TrypLE One sterile 15 ml conical tube
Making Mammalian Cell Growth Medium 1. The cells that you are working with require the following constituents in the growth medium: 10 mM glucose 4 mM glutamine 1 mM pyruvate 8.3 g/L DMEM 3.7 g/L NaHCOз 10% FBS 2. Take time to calculate the appropriate amount of each of the above items to prepare 100 ml of growth medium using the prepared liquid stock reagents. ***Check with your laboratory instructor before proceeding.
Transcribed Image Text:Making Mammalian Cell Growth Medium 1. The cells that you are working with require the following constituents in the growth medium: 10 mM glucose 4 mM glutamine 1 mM pyruvate 8.3 g/L DMEM 3.7 g/L NaHCOз 10% FBS 2. Take time to calculate the appropriate amount of each of the above items to prepare 100 ml of growth medium using the prepared liquid stock reagents. ***Check with your laboratory instructor before proceeding.
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