Label three sets of eight microfuge tubes (1-8) and add the volumes of BSA stock solution (2 mg/ml) and water to each labeled tube as indicated in Table 3. Be sure to calculate, and enter, the values for column 4 of this table. Table 3 STD Curve Volume BSA Volume Water (µ) Final BSA Concentration tube (mg/ml) Stock (ul) number 100 2 5 95 3 10 90 4 20 80 5 40 60 6 60 40 7 80 20 100 00
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- If you had a cell suspension of 2.5 x 10^4 cells/mL and plated 100uL into each well (4 wells), how many cells were added per well?The doctor ordered Oxacillin 650mg every 6 hours. The instructions on the 2gm vial states to reconstitute with powder with 11.5ml of sterile water for injection, yielding 220mg/1.5ml. How many ml will you administer? using dimensional analysis and show workHow much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? V1= 0.005mL B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?
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- When the yeast cells have completely re-hydrated, measure out 1.8 mL of well-mixed yeast suspension (0.2% yeast) into each of two new 15 mL centrifuge tubes. Add 200 μL of a 10% (w/v) Sodium Azide*** in YGM solution to one of the 15 mL tubes with yeast suspension. Add 200 μl of YGM to the other 15 mL tube of yeast suspension for your Control. Let both tubes incubate at room temperature for 30 min, vortexing every ~5 minutes What is the % concentration of azide in this sample during metabolic inhibition?What is the % concentration of yeast in this sample during metabolic inhibition?Your pharmacy has on hand Tetracycline 250 mg/5 mL liquid.You receive a prescription for Tetracycline syrup 500 mg qid for 7 days. How many total milliliters would you dispense? Your pharmacy has on hand Prednisone 5 mg tablets.You receive a prescription for Prednisone 5 mg ii tabs stat and qid x 1 day, then ii tabs tid x 2 days, then ii tabs bid x 2 days, then i tab tid x 3 days, then i tab bid x 3days, then i tab qd x 4 days How many tablets would you dispense for this prescription? How many days will the patient take this medication for? Your pharmacy has on hand Dilantin 100mg capsules.You receive a prescription for Dilantin 100 mg 1 cap po in the AM & PM, 200mg (2 caps) po at noon, and 300mg (3 caps) po at hs. Dispense a 30 day supply. (using the 100mg capsules you have in stock) How many capsules will you dispense? Your pharmacy has on hand Dakins Irrigation Solution 1 liter.You receive a prescription for Dakins Irrigation Solution Irrigate chest tube with 100 mL…Using 25 uL of a patient's serum, how much diluent would be needed to create a 1:2 dilution? ______ ul