Q: Which is a limitation of using massively parallel sequencing ?
A: It is a term used to describe the method of high-throughput DNA sequencing to determine the entire…
Q: What are the five main steps in DNA cut-and-paste transposition?
A: The "cut and paste" method is used for saving conversion. The enzyme transposase acts as a pair of…
Q: What would be some major impediments to genetically modifyinghuman embryos with CRISPR?
A: Genome editing tools are those tools which help in making changes into the genome of an individual.…
Q: How to do linear Restriction Mapping of gene fragments, including a combination of three enzymes?
A: Bacteria contain restriction enzymes. To destroy viruses, bacteria use restriction enzymes, which…
Q: Name a special enzyme used by a "copy and paste" transposable element.
A: Transposable elements Transposable elements are sequence found in DNA that have ability to move…
Q: Why is it difficult in a single experiment to transfer a largenumber of genes to a recipient cell…
A: Gene transfer is the process of insertion of unrelated DNA into cells. There are three mechanisms of…
Q: What is the useful of the Development of highly sensitive and low-cost DNA agarose gel…
A: In today's medicine, extremely sensitive and low-cost DNA agarose gel electrophoresis is widely used…
Q: Describe step 1: hybridization of template and primer in automated sanger sequencing
A: Introduction Sanger sequencing is also known as the “chain termination method”. It is a method for…
Q: What are the advantages of using a restriction enzyme whose recognitionsite is relatively rare? When…
A: Restriction enzymes or restriction endonucleases are enzymes capable of recognizing a specific…
Q: What does CRISPR-Cas stand for and how does it work? What are the molecules involved with CRISPR-Cas
A: Introduction Genome editing:- It is also known as gene editing or genome engineering. It is a type…
Q: What is the significance of palindromic Restriction Endonuclease recognition and sequence overhangs…
A: Restriction endonucleases enzyme is also known as molecular scissors, which helps in cleavage of DNA…
Q: What are the possible bioethical issues that gene editing tools may encounter?
A: Gene editing tools are used to edit an organisms genetic code , i.e.. with these tools ,the genetic…
Q: What are three benefits Ion torrent sequencing has over sanger sequencing
A: DNA is an important biomolecule in the cell as it is the genetic material for most of the organisms…
Q: What is CRISPR-Cas9? What is one advantage and one disadvantage of this technology?
A: Gene editing is a technology in which Genetic material of an organism is altered and remodelled.…
Q: What are the vectors used in rDNA technology? briefly explain each
A: rDNA technology or Recombinant DNA technology is a widely used technology with the advancement of…
Q: What is Western blotting ?
A: Blotting refers to a technique by which a biomolecule is transferred from one medium to another…
Q: What is the difference between using genome build hg18 and hg19 in Genome-wide SNP arrays?
A: SNP (Single nucleotide protein) array is a type of DNA (deoxyribonucleic acid) microarray which is…
Q: What is it about the Cas9 protein that makes it an efficient DNAediting tool?
A: DNA and RNA are the genetic elements present in almost all cells. DNA encodes for proteins through…
Q: Which vectors can be used to clone a continuous fragment of DNA with the following lengths? a. 4 kb…
A: According to the question, we have to mention the name of the vectors that can be used to clone a…
Q: What is linear amplification PCR?
A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…
Q: How can linkage disequilibrium mapping sometimes provide a much higher resolution of gene location…
A: Linkage analysis rely on co inheritance of functional polymorphism, In linkage analysis, there are…
Q: Distinguish between a Northern, Southern and Western blot
A: Southern blotting is a blotting process for detecting particular DNA sequences, Northern blotting is…
Q: What is restriction fragment length polymorphism and which method do we use to detect it?
A: Restrictions length polymorphism (RFLP) is a kind of polymorphism that outcomes from variety in the…
Q: What are the advantages of Next Generation Sequencing?
A: DNA sequencing is a biochemical method for determining the order of nucleotide bases, A, G, C, and…
Q: Short tandem repeats (STR) profiling is based on
A: Small tandem repeats (STRs) are short repeating DNA sequences (2–6 bp) that make up roughly 3% of…
Q: What is the role of the following molecules in the CRISPR mechanism? *
A: CRISPR stands for clustered regularly interspaced short palindromic repeats. These are a set of DNA…
Q: Why is it necessary to boil the probe before you use it in your southern blot?
A: Southern blotting is a molecular biology technique that is used to detect a person's DNA. This…
Q: Give a step by step method and diagram of how reversible terminator sequencing works.
A: The reversible terminator sequencing or Illumina dye sequencing is a technique used to determine the…
Q: CPT code assignment 44970
A: Cpt code is current procedural terminology. These are the numerical codes helps to identify the…
Q: Why does reversible chain termination sequencing yield short reads?
A: In genetics, sequencing is considered a methodology to determine the order of nucleotides(adenine,…
Q: Briefly Explain different applications of western blotting. Please explain at your own words.
A: A western blot is an analytical molecular biology laboratory method that is used in the detection of…
Q: Gel Electrophoresis Separates DNAFragments According to which thing?
A: Electrophoresis is a technique used for separating the components of a mixture of charged molecules…
Q: What is an advantage of RNA Seq over microarrays?
A: The process in which the sequencing of total messenger ribonucleic acid (mRNA) is carried out…
Q: What is CRISPR?
A: Introduction Bacteria and viruses are tough competitor to each other, in order to overcome each…
Q: What is the difference between a map-based approach to sequencing a whole genome and a whole-genome…
A: Deoxyribonucleic acid (DNA) is a double stranded helical genetic material containing thousands of…
Q: What is the major difference between the strategies of map based sequencing and shotgun sequencing?
A: DNA sequencing is a process in which sequence(order) of nucleotides in DNA is determined. It…
Q: What are frame-shift insertion?
A: Mutations are changes that occurs in the deoxyribonucleic acid (DNA) sequence, either due to…
Q: What is Northern blot analysis ?
A: The nucleic acids transfer on a solid phase membrane for identification is called the blotting…
Q: Describe the features of a typical CRISPR locus in a bacterial cell.
A: CRISPR (short for clustered regularly interspaced short palindromic repeat) acts as the immune…
Q: How else can it be proven that transformants have the topA-cysB fragment apart from size analysis…
A: A transformant cell is a cell that has received additional genetic material, either experimentally…
Q: What is the difference between bulk RNAseq and single cell RNA seq?
A: In RNA sequencing, RNA is taken out from tissues of different cell types for analysis of RNA seq .…
Q: what are the advantages and disadvantages of CRISPR cas9 technology?
A: CRISPR -Clustered Regularly Interspersed Short Palindromic Repeats The CRISPR cas9 is a technology…
Q: What are the main steps of Western Blotting?
A: The technique of Western blotting is a well-known and commonly used method for detecting and…
Q: CRISPR
A: Interestingly, CRISPR-Cas9 could be used to the investigation of treatments of various human…
Q: what is the purpose of calcium chloride in the transformation process
A: Calcium chloride transformation is a laboratory technique in prokaryotic (bacterial) cell biology.…
Q: For the hybridization step in the Southern blotting, the hybridization was done at 85 degrees…
A: Southern blotting is the most common molecular biology technique. A Southern blot is defined as a…
In Northern and Western blotting, what is the purpose of gel electrophoresis?
Step by step
Solved in 2 steps