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- In the conduct of serial dilution, which of the following tools is NOT necessary? a. Agar plates b. Micropipettor c. Inoculating needle d. Alcohol lamp or Bunsen burner e. Test tubes or microcentrifuge tubesExplain why the following steps are essential during subculturing:a. Flaming the inoculating instrument prior to and after each inoculation.b. Holding the test tube caps in the hand as illustrated in Figure 1.1 on page 24.c. Cooling the inoculating instrument prior to obtaining the inoculum.d. Flaming the neck of the tubes immediately after uncapping and beforerecappingThe following methods used for sterilizing an antibiotic solution. i. Dry heat sterilization ii. Microfiltration iii. Autoclaving iv. Desiccation Select one: O a. i and ii O b. i and iii O c. ii and iv O d. ii only
- Choose the combination of answers that most accurately completes the statement. Detergents are a. high-level germicides c. excellent antiseptics b. low-level germicides d. used in disinfecting surgical instruments followingMatch the test with the appropriate reagent/ chemical a. DNA test b. Paper chromatography c. Cellular respiration d. Enzyme testChoose the combination of answers that most accurately completes the statement.Detergents are a. high-level germicides c. excellent antiseptics b. low-level germicides d. used in disinfecting surgical instruments
- Which one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? Select one: a. Agar macrodilution method b. Gradient diffusion method c. Broth microdilution method d. Disk diffusion methodWhich type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus pyogenes from a patient’s throat swabb. isolating a pathogen from a patient’s clean-catch urine samplec. isolating enteric bacteria such as Escherichia coli from a sample oforganically grown spinach d. maintaining a patient’s nasal swab specimen for further analysis andidentifi cation of possible respiratory syncytial virus (RSV) infectionWhich of the following would be best for sterilizing a pre-sealed case of plastic medical supplies? A. Autoclave B. Ionization Radiation C. Sonication D. Nonionizing Radiation
- Which of the following methods is most sensitive for identifyingdifferent strains of a microbe?a. microscopic examination b. hemolysis on blood agar c. DNA analysis d. agglutination testChoose the combination of answers that most accurately completes the statement. The most versatile method of sterilizing heat-sensitive liquids is a. UV radiation c. beta propiolactone b. exposure to ozone d. fi ltrationNeutralization of residual disinfectant could be made by: a. Dilution if the concentration exponent is high b. Dilution if the concentration exponent is low c. Adding specific agent e.g. 3% Tween 80 d. Either a or c e. Either b or c
