Explain how PEMBA (polymyxin-pyruvate-egg yolk-mannitol-bromthymol blue-agar) is used to isolate, differentiate and enumerate Bacillus cereus from food sample.
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Explain how PEMBA (polymyxin-pyruvate-egg yolk-mannitol-bromthymol blue-agar) is used to isolate, differentiate and enumerate Bacillus cereus from food sample.
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- Selective and Differential Conditions of media and culture conditions can be used to culture and identify Listeria monocytogenes. For each, characterize it as Available Hint(s) Res Adding antibiotics, such as nalidixic acid Adding high concentrations of salts, such as lithium chloride and sodium chloride Adding a pH indicator to detect fermentation of hamnose to acid Incubate at 30°C Detecting beta-hemolysis Adding antifungals, such as amphotericin B Differential SelectiveMake the Dichotomous key for M. luteus, Bacillus subtilis, bacillus, Megaterium, Bacillus cereus, E.coli, S. Marcescens, and Enterobacter aerogenes based on catalase test, (lactose, sucrose and glucose fermentation tests) and other biochemical test for accurate identification and colony morphology.Describe the appearance of an S. agalactiae colony grown on blood agar. Describe how that colony would differ in appearance from a colony of S. pyogenes.
- Vibrio cholerae can be cultured on Thiosulfate-Citrate-Bile salts-Sucrose agar (TCBS). Enterobacteriaceae and gram-positive bacteria are inhibited on this agar. cholerae produces large yellow colonies while other Vibrio species produce blue, green, or blue-green colonies. Discuss the purpose of each media listed. Based on this information alone, what term(s) would best describe this agar? Selective Enrichment (enriched) Differential Reducing Selective and enriched Selective and differential Selective, differential, and enrichedIdentify a biochemical test that can differentiate Enterobacter agglomerans from Enterobacter cloacaeDescribe the selective and differential properties of mannitol salt agar (MSA) for the isolation and identification of staphylococci.
- Why is glucose not the choice of carbohydrate for most differential media used in the identification of gram (-) enteric bacilli?Explain why the media where the Staphylococcus aureus is plated has turned yellow but the media where Staphyloccocus epidermidis plated is still pink? The media used would be the Mannitol Salt Agar Medium.How would you distinguish between Listeria monocytogenes and othe Listeria species on Chromogenic Listeria agar supplemented with Brilliance Listeria Differential supplement (SR0228)?
- Hypothetically make the Dichotomous key for Micrococcus luteus, Bacillus subtilis, Bacillus, Megaterium, Bacillus cereus, E.coli, Serratia Marcescens, and Enterobacter aerogenes based on colony morphology. Starting from the results of gram staining, catalase tests, (lactose, sucrose and glucose fermentation tests) and other biochemical tests for their accurate identification.Identify the oxidase-negative, gram negative bacillus isolated from the blood cultures of a hospitalized patient with a urinary tract infection. The organism produced colorless colonies on MacConkey agar and gives the following biochemical reactions: PAD – positive H2S – positive positive Citrate – positive Ornithine positive Lysine – negative Indole - negative Urease –Why is it necessary to perform an enrichment procedure in isolating Staphylococcus aureus instead of directly plating it into a selective medium? What might happen if you omit potassium tellurite in using GCM?