3. The /C50 value for a drug called razundib for a Src kinase is 15 nM. The Src kinase isknown to bind ATP with a dissociation constant (KD) of 0.05 mM. If the concentration ofATP in the cell is 0.75 mM, what is the value of K, for rasundib with respect to the Srckinase?

Src kinase is a tyrosine kinase that catalyses the phosphate group transfer from ATP to the tyrosine…

Answered: 3. The IC50 value for a drug called…

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter32: The Reception And Transmission Of Extracellular Information

Section: Chapter Questions

Problem 21P

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2. The two diagrams to the right il- lustrate plots of steady-state ki- netic studies to characterize the in- FB 0.8- nH = 3.5 0.6- teraction of heart muscle phos- phofructokinase-1 with a non-phy- siological, synthetic substrate fruc- tose-6-sulfate. Because the kcat is smaller than that for the natural 0.4- 0.2- 10 μΜ 20 μΜ 48 μΜ substrate, higher enzyme concen- trations could be used. The results show the influence of increasing 2- 3.2- 0.4- concentrations of ATP on the initial -0.6- > velocity of the enzyme catalyzed reaction in the presence of no AMP (•), 10 µM AMP (•), 20 µM AMP (-), and 48 µM AMP (-). -0.8- 4 12 20 28 36 44 52 60 68 76 84 92 1.2 1.6 2.0 2.4 [AΤP (μΜ) log[ATP] (µM) (a) how ATP interacts with the enzyme in the case of no AMP (•). Write the reaction in words catalyzed by the enzyme for the alternative substrate, describe (b) with respect to the binding of AMP and ATP to the allosteric effector sites on the enzyme. Explain the physical significance of the…

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3. The IC50 value for a drug called razundib for a Src kinase is 15 nM. The Src kinase is known to bind ATP with a dissociation constant (KD) of 0.05 mM. If the concentration of ATP in the cell is 0.75 mM, what is the value of K₁ for rasundib with respect to the Src kinase?