2. What would the total magnification be if a microscope had 15X ocular lenses and 4X, 10X, 45X and 95X objective lenses? Insert the correct information in the following table. Magnification of Objective Lens Magnification of Objective Lens Total Magnification Ocular Lens 4X Objective lens 10X Objective lens 45X Objective lens 95X Objective Lens
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- What is the DFV of a 40x objective lens if the DFV of a 10x objective lens is 3 mm? Express your answer in μ (micra)5)The simulation asks you about the TOTAL magnification of when you use the 40x objective. This total magnification is based on the the amount of magnification provided by the ocular lens in the eyepiece, as well as the magnification of the objective. Based on the answer given in the simulation, what is the magnification of the ocular lens? (enter the number only) Answer valueWhich of the following affect the resolution of a specific objective lens? CHECK ALL CORRECT CHOICES. Magnification Numerical Aperture (NA) Warelengthrofgnt combg tough t Field of View
- If you have chosen the objective lens with 10x and the ocular lens magnification is also 10x. Then the total magnification will be 100x. True FalseIn this image a stage micrometer and an ocular micrometer are aligned so that the 10 o.u. line aligns with the 100um line. The 200um line of the stage micrometer overlaps with the 90 o.u. line of the ocular micrometer. What is the calibration for this lens? eyepiece graticule 40 50 60 70 s0 9 100 stage micrometer 0.8 um/ou O 8 um/ou O 1.25 um/ou 0.25 um/ouIf the ocular lens is 10x, what would be the total magnification for the following objectives lenses? 5x 17x 35x
- A michroscope has a 20x ocular and two objectives of 10x and 43x respectively: calculate the apparent magnification for low and high power lenses.Using the Field of View (FoV) formula (Low Magnification/High Magnification)= (High Field of View/Low Field of View) determine the FoV in microns for all other objective lenses listed in yourtable given that the diameter of the 4X scanner FoV is 3.2 mmTable 1. Objective Lens Scanner Low Power Objective High Power Objective Oil Immersion Objective Total Magnification Objective magnification Ocular magnification Procedure: 1. Locate and record the numbered X in the ocular and the objectives. These numbers represent the magnifying ability of the microscope lenses 2. To compute the magnification, get the product of the magnifying power of the objectives and the eyepiece. Reflect your results in Table 1.3
- Calculate for the calibration factor. (3 points) (Note: stage micrometer is 1 mm long with 100 divisions) EYEPIECE RETICLE 10 20 30 40 50 60 70 0 80 100 M⁹⁰ př 10 40 50 20 OPE www.fanfi |||||||| STAGE MICROMETER 60 |||||||Compute for the total magnification with the different objectives. Fill in Table 2 below. Table 2. Total magnification per objective. Mocular 10X 15X Scanner 4X LPO 10X HPO 40X ΟΙΟ 100XUse the iris diaphragm to attain the best illumination of the letter “e” on the scanning (4x) objective. Open and close the iris diaphragm and notice the changes in contrast of the letter “e”. How did the appearance of the letter “e” change under the various levels of illumination? 1 Repeat the above procedure on the high-power (40x) objective. When increasing magnification, what happens to the light available? Why?