2. Given the results table below, create a flow chart to differentiate the organisms so you could identify an unknown isolate out of these four possibilities. Organism Urease MR TSI Glucose fermentation JM02 + A/H₂S+ A SL65 + A/A Ag DT22 K/K K YS98 + - A/A Ag
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- 8. A. Use Excel (or another graphing program) to draw the growth curve, In (X/X.) vs time, for bacteria grown in a 20 L suspension cell culture, given the following data: - initial concentration: 0.120 gdw cells/L Also report: - lag time: 1.5 hours - mass doubling time during exponential growth: 250 minutes - duration of exponential growth phase: 1 day (24 hours) - negligible time in the deceleration phase - 13 hours in the endogenous metabolism phase with no change in cell concentration - cell death rate with k = 0.0178 min -¹. B. What is the specific growth rate, µ? C. What is the maximum concentration of cells in the reactor? (gdw cells/L) and when does this occur? D. Other than time zero or the end of lag phase, at what time is the concentration of living cells in the reactor equal to the initial concentration of 0.120 gdw/L?N. benthamiana will be infiltrated with a solution containing OD600=0.3 of each experimental Agro containing construct and OD600=0.1 of p19. Calculate the volume of cultures (V construct; V p19) needed according to the formulas: V construct = V final × 0.3/OD600; V p19 = V final × 0.1/OD600. One mL of infiltrate is often enough to complete a small experiment. How to plan your final volume accordingly?Which of the following is the a-anomer? H но H Но H2C H ОН H2C- нн ОН со 4 1 2 1 Submit 3 -OH Н ОН -OH ОН Н there is no a-anomer ОН ОН Н Request Answer H Но НО Н H2C нн ОН 2 H2C ОН -OH 4 Н ОН н н H OH -О. ОН ОН Н ОН Н
- Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?1. Provided with the following data, compute the corresponding CFU/ml of the original culture. Assume that spread plate technique was done. Show your solutions. 10 10 10 10* 10 Plate 1 TNTC 340 132 45 19 Plate 2 TNTC TNTC 242 80 25 Plate 3 TNTC 280 90 22 2 2. If a broth culture of bacteria, initially containing 37,000 bacteria/ml, were diluted to a 1:1,000 dilution, how many bacteria/ml of the diluted broth would be present on average? Show your solutionsThe zone of inhibition measurement for S. aureus with an antibiotic disc of erythromycin is 10. Looking at the chart below, would you say the bacteria is resistant, intermediate, or sensitive/susceptible to the antibiotic? Resistant Intermediate Susceptible Penicillin G (10ug) S28 229 Oxacillin (1ug) S10 11 -12 213 Erythromycin(15ug) S13 14-22 223 Gentamycin (10 ug) S12 13 -14 215 Tobramycin(10 ug) <12 13 -14 215 O Intermediate Resistant O Sensitive/Susceptible
- Three different strains of Escherichia coli, respectively A, B, and C have been inoculated in three different media, namely I, II, and III, and the growth of each bacterial strain has been followed by spectrophotometry (Figure 1). Strain A Strain B Strain C 4.5 45 Medium I 4 3.5 25 2.5 3 E 2.5 Minimal medium + Glucose 25 2.5 O 15 1 0.5 as 05 100 200 300 400 500 600 100 200 400 600 200 Time (minj Strain A Strain B StrainB 4.5 4s 4s Medium II 3.5 15 25 2 3 Minimal 25 2 medium + Glucose 1 + Arginine 0.5 as as 100 200 300 400 500 600 100 300 400 s00 600 100 300 400 600 Tme Time (min) Strain A me StreinB Streine 4.5 45 45 Medium II 4 4. 3.5 3.5 25 2 3 Minimal medium + Glucose 5 2.5 25 25 8 15 15 + Lactose 1 0.5 05 05 100 200 300 400 500 600 300 200 400 10 200 300 630 Time (min) Tine imin Time min Figure 1. Growth curves of the three E. coli strains A, B and C in three media: I, minimal medium containing glucose; II, minimal medium containing glucose and arginine; and III, minimal medium…of 1 stion s page 30- F2 Which of the following terms is associated with the probability of a preparation not being sterile after it has been exposed to a sterilization process? Select one: Oa. Particulate Matter level Qb. Sterility Assurance level О с. Pyrogen Load level O d. Optimal Sterilization level F3 F4 F5 Ol پیار Q Search F6 F7 F8 F9 === 8 9 F10 Time left 22:34:03 F11 Next pageWhich can infect hur Table 1: Absorbance and appearance of broth tubes at various temperatures. E. colin B. stearothermophilus Temperature°C Absorbance Visual Absorbance Visual 4 0.04 Clear 0.06 Clear tre Light turbid Very turbid Almost clear 25 0.66 0.09 Clear Light turbid Very turbid 37 1.48 0.55 which 55 0.25 1.29 oledzethe mes he alhe ive to edeut et brahell bras atnstooinieib o ho leviviue zwolls ti saun
- Why the synergy test results demonstrated that Penicillin G and Gentamicin did not act synergistically against the Streptococcus group G strain? Reference: https://drive.google.com/file/d/1nphMwenA3HSR_BjX5bzwQNYM0qbuqDdh/view?usp=sharingBased on prior experiments, you have determined that for a culture of S. aureus an absorbance reading of 1.0 AU650 is approximately equal to 7.25 x 106 cells/mL.How many cells should be present after 10 generations in a 1 mL culture, if the initial cell culture had an absorbance reading of 0.5? (3 Points)You have several different media onto which you inoculated eight strains of yeast (A-H). The media include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin. After overnight incubation, the following results were observed (tan patches represent growth): D plate 1 (A) B DE F GH plate 5 plate 4 plate 6 Which plate contains an unsupplemented minimal medium? [Select] Which plate contains a rich medium? [Select] plate 2 Which strain is a prototroph? [Select] Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy? [ Select] plate 3 plate 7 One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to be? [Select]