Lab-7-Control-of-Microbial-Growth

Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture   1. Microscope     2. Colony Counter     3. Autoclave     4. Microbiology incubator     5. Drying oven     6. Refrigerator (microbiology)     7. Bunsen burner/alcohol lamp     8. Candle jar     9. Anaerobic jar     10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet     11. Bacteriologic filters (Seitz, Chamberlain, Berkfield)     12. Petri dish     13. Culture tubes     14. Hanging drop slide     15. Durham’s tube     16. Staining rack     17. Thermostatically controlled water bath     18. Inoculating loop     19. Inoculating needle     20. Vials

Instruction: answers must be in numbers. You are culturing bacteria using a petri dish, the bacteria grow very well on this plate you have. However, your microbiology instructor wants to know how many bacteria per milliliter (bacteria/mL) are on your plate. And as you have remembered during the first day of inoculating this bacteria you used a imL aliquots sample of a 1:10,000 dilution, and the total colony you counted on this plate is 170. How many bacterial per milliliters would that be?

answer the following questions with book reference. 1. What is meant by a bacterial “colony” or colony-forming unit? 2. Why are colonies that develop on a heavily seeded plate smaller than those that appear on a sparely seeded plate? 3. What are some of the uses and advantages of the ff.:a. Agar plateb. Agar slantc. Broth culture 4. What are the advantages and limitations of studying bacteria by means of the Culture method? 5. Why is it desirable that most cultures be inspected after 15 to 18 hours of incubation? 6. Why should culture media after inoculation be incubated at an optimal temperature immediately? 7. Describe other types of aerobic jars and anaerobic methods.

1:07 < Вack Untitled 6 Brownian movement of bacteria: O a. Can be observed by the of soft agar technique O b. Can be observed by staining of Staphylococcus aureus Oc. Is common in Escherichia coli or Proteus vulgaris O d. Is common in Staphylococcus aureus O e. Can be observed at the edge of a microscope CLEAR MY CHOICE

D Question 37 Based on the data below, how much more UV resistant was B. licheniformis relative to S. aureus? (All controls had growth.) - covered with lid Organisms Exposure 20 sec Staphylococcus aureus 5 sec + 20 sec Bacillus licheniformis + 10 sec 80 sec times to UV light 40 sec 2.5 min 160sec 5 min 10 min 5 min 20 min 10 min 40 min 10 min + 40 min

General Instructions:Choose 1 bacteria/diseases and fill out the details. An example of an answered template is also provided for your reference.    Causative Agent and Disease Profile for S. aureus Template and Example   ITEM MSM PROFILE   MICROBIAL PROFILE   I MICROORGANISM/CAUSATIVE AGENT Staphylococcus aureus A GRAM REACTION (+) B OXYGEN REQUIREMENT Facultative Aerobes C SIZE 1.5 µm D SHAPE Cocci in clusters E HABITAT Normal flora of skin/anterior nares/pharynx F DISCOVERY   G MICROSCOPIC IMAGE   II DISEASE PROFILE Scalded skin syndrome A DISEASE/S                        Skin and Wound Infections       Scalded Skin Syndrome Toxic Shock Syndrome Food Poisoning Pneumonia B SYMPTOMS OF THE DISEASE A high fever · Nausea and vomiting · A rash on your palms and soles that resembles a sunburn C INCUBATION PERIOD 2 and 4 hours (range 30 minutes to 8 hours) D MODE OF…

Question:- Describe control strains used in the clinical microbiology laboratory and explain their maintenance in the laboratory. ( write BY WORD and all steps I need). Introduction Discussion References

The student advises using the same selection procedures and inoculating a 5 mL overnight culture with the transformed cells instead of searching for transformed coli on LB agar plates in order to save time. Why isn't this a wise decision?

explain how the following genotypic andphenotypic methods of microbe identification is done. Use diagrams or schematic representations to explain yourwork.  1.Gram Staining2.Biochemical Reactions: Multiple Tube Fermentation Technique3.(Biochemical Reactions: IMViC Test

MICROBIOLOGY:  Microscopic Morphology of Microbes Write your introduction (This includes principles, significance of the study, objectives of the experiment and how the objectives were achieved. This part must also be in the passive voice and past tense. Introduction must be short but packed with relevant content). another: What is the advantage of the Gram stain over the simple stain? What is the theory about the mechanism of the Gram-stain reaction?

Question:- How to perform a bactericidal test bactericidal test of Dental Pulp Stem Cell (DPSC)?

Step #1 Culture and perform a Gram-stain on the Super Bug You culture the Super Bug by swabbing the patient's wound, applying the sample to the surface of an agar plate (petri dish), and incubating the plate for one day. Next you perform a Gram stain on a pure culture of the Super Bug cells. 1. Considering the bacteria were originally isolated from an exposed wound on a human patient, under what conditions (temperature, atmosphere) did you most likely incubate your plate of Super Bug cells to achieve maximal growth? Gram stain observations: Short rod-shaped cells found in clusters, stained red/pink 2. Is the Super Bug Gram negative or positive? 3. What conclusions can you make about the components of the Super Bug cell wall?

Please match the test with its description to assess your understanding of the kinds of data collected during information gathering and your understanding of the processes involved in identifying microbes from samples. 1. using macroscopic and microscopic traits for identification appearance 2. tests that determine chemical characteristics including enzyme production and nutritional requirements of the microbe biochemical tests 3. analyzing the genotype of an organism DNA profiles 4. tests the organism against known antibodies to determine if there is a reaction between the organism and the antibody immunologic testing v

Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture   1. Microscope     2. Colony Counter     3. Autoclave     4. Microbiology incubator     5. Drying oven     6. Refrigerator (microbiology)     7. Bunsen burner/alcohol lamp     8. Candle jar     9. Anaerobic jar     10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet

Sample Station 7 A. If a student performs a Gram stain on a Gram negative bacterium and stops the stain technique after the iodine step and rinse, what color would you predict the cells to be if you looked at it right now under the microscope?

1. Describe the three different type of hemolysis that are observed on blood agar.2. What is a selective medium?3. What is a differential medium?4. Which media can be used to isolate E. coli samples from contaminated lettuce?5. Which two media would be used to identify a sample taken from a patient with suspected gonorrhea?6. Would you be able to grow a sample obtained from a patient's wound (suspected to be infected with MRSA) on EMB? Explain.7. What is the color or TSI for Salmonella?8. What is a fastidious organism?

ISOLATE #2 Results for "Isolate #2": ***Please fill out the table**** 1. Blood agar: 2. Nitrate Test: Test Results Interpretation Blood agar (type of hemolysis) Nitrate test 3. Gelatinase Activity: Gelatinase activity Resistant or Susceptible? Novobiocin Resistance Zone Diameter: 4. Novobiocin Resistance: 5. Coagulase Test: Coagulase (Sure-vue test) 2. Based on your results, what is the possible identification (Genus and species) of "Isolate #2"? nhes

LABORATORY REPORT: Aseptic Technique & Inoculation of Bacteria Guide Questions. 1. Why is direct flaming preferred when disinfecting loops and needles? 2. Why is it important to flame the entirety of the loop and not just the tip? What consequences can be seen when this process is not done correctly? 3. What is the difference between quadrant streak method A from method B? 4. Why do we use a pencil instead of a pen when labelling culture tubes or plates? Are there other alternatives in labelling? 5. Why is an inoculating needle used in Subculturing Techniques? Can a loop be used instead? NOTE: Please try to answer all of the question asked, i promised to give you a good ratings

ISOLATE #1 Results for "Isolate #1" ***Please fill out the table**** 1. Blood agar: 2. Nitrate Test: Test Results Interpretation Blood agar (type of hemolysis) Nitrate test 3. Gelatinase Activity: Gelatinase activity Resistant or Susceptible? Novobiocin Resistance Zone Diameter: 4. Novobiocin Resistance: 5. Coagulase Test: Coagulase (Sure-vue test) 1. Based on your results, what is the possible identification (Genus and species) of “Isolate #1"? I 9 10 11 12 3 14

Subculture and Colony Morphology Descriptions Following identification of the Gram-negative isolate and Gram-positive isolate, you next subculture each onto fresh nutrient agar plates.  Briefly describe the subculture process in three or so sentences and what this allowed you to achieve; follow this with colony morphology descriptions of each.    Description:           Isolate A – Describe:    Colony morphology:    Medium & incubation temperature: Isolate B – Describe:    Colony morphology:    Medium & incubation temperature:

IN YOUR OWN WORDS. Differentiate the following. (Not more than 10 sentences) 1. Aseptic Teachnique vs. Sterile Technique 2. Microbistatic Agent vs. Microbicidal Agent 3. Sterilization vs. Disinfection 4.How is radiation, autoclave and ultrasonic waves used in the hospitals or clinics as a means to inhibit the growthbof microbes? 5. How is filtration applied nowadays in relation to Covid-19?

BONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J K

microbial sensivity lab: in the procedure used to test bacterial growth against various temperatures (incubator, room, refrigerator, freezer), why should efforts be made to inoculate each tube with the same number of bacteria?

Smear Preparation and Staining Lab Smear Preparation 1. What are two goals of a good smear? 2. Why does a bacterial smear need to completely air dry prior to heat fixing? 3. What is the purpose of heat fix step? Simple Stain 1. What is the difference between simple stain and complex stain? 2. Describe the color of an S. aureus after performing simple stain with Safranin Red. Why? 3. What do you conclude after performing simple stain and observing "Purple cells longer than they are wide Gram Stain 1. How does the bacterial cell structure contribute to the mechanism of Gram staining? 2. What is the mordant in Gram stain and why you need it? 3. Why must young cultures be used when doing a Gram stain? Negative Stain 1. а. Name the bacterium used in negative stain b. Describe morphology С. Name the disease caused by this bacterium 2. Describe what you observe under microscope in negative staining.

A student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 Celcius

Please help me answer correctly the table this is my assignment in biochem lab Procedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 40°C , the 4th tube at 60°Cwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict’s tests.

1. If you expose E.coli to UV light at 30 seconds, 1 minute, 3 minutes and 5 minutes, what are your expected results? 2. If you expose. B. cereus to UV light at 30 seconds, 1 minute, 3 minutes and 5 minutes, what are your expected results? 3.  If you do not remove the cover of the plate before exposure to UV light at 5 minutes for E.coli and B. cereus, what are your expected results? Help with 1,2 & 3 please

Discusses the methods to -1 optimise fluorescent cellular (staining (priority first List the freezing -2 parameters of the slow cooling technique Parameter of verification -3 precedure of freezing 9

(1) why can't we say "sterile" technique (2) how are aseptic technique similar and different in the lab and Healthcare field?Be specific and explain at least 2 differences and two similarities.  (3) You are asked to develop a method of transfer an unknown organism from a liquid  broth to a solid petri dish.list each step that you would have to take .be specific

have a progressive su week. 1. Why are basic stains the most commonly used stains in microbiology? 2. What does the term "aseptic" mean? 3. Why are differential stains used more often than simple stains? 4. What are the three parts of a stain? 5. Why is it critical to allow cells to air dry before heat fixation? 6. Give an example of a basic stain and an acidic stain. 7. Give an example of a differential stain. 8. What is a medium? 9. What is the definition of a bacterial colony? 10. Why do we want to get an isolated bacterial colony from a mixed culture? Next lab: A continuation of staining techniques. to a

indicators, key used for/ determines reagents, or key positive result ingredients 1. crystal violet Comments/ additional info Medium/test negative result decolorization is key. Must include +/- controls on gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink 3. gram's declorizer 4. safranin shape every slide to verify result quadrant streak- isolation of pure culture of bacteria check this for any pigmentation of colonies TSA Eosin methylene blue agar (EMB) 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. blackening of medium 2. red color in added reagent 3. fuzzy appearance of 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. ferrous salts 2. Kovac's SIM in one medium) 3. low % agarose medium/stab not visible motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red fermentation broth test media (list the specific…

Worksheet 2. Draw it. Instruction: A bacterial culture was in log phase. At time x, an antibacterial compound was added to the culture. Draw the lines indicating addition of a bacterial compound and a bacteriostatic compound. Explain why the viable count does not immediately drop to zero at x? Time Log of number of cells

I need help with microbiology What is the magnification of each of the following objectives? scanning:   low power:   high dry:   oil immersion:

Identification of Gram-positive cocci: You have isolated a catalase-negative bacterium that doesn't grow well in 6.5% NaCl medium. Which test would you expect to give you a presumptive identification? (i.e. The first tests tell you the genus, which test would identify a species of that genus)   CAMP Test   Coagulase Tube Test   PYR Test   Novobiocin Sensitivity Test