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- When performing a negative stain, how does it work and why does it only stain the background?a: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.Differentiate between the “hot method” and “cold method” of acid-fast staining.