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Q: Microbiology
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Q: Differentiate between negative and positive staining, givingexamples.
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Q: the steps in doing gram staining using a flow chart.
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Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle, procedure and results.
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- write the protocol for Hexamine silver staining, including their reagents, materials, prinicple, procedures results, and cautionsWrite down the materials, reagents, Principle, procedure and results for Ziehl-Neelsen stainwrite the protocol for mann's methyl blue-eosin staining, including their reagents, materials, prinicple, procedures results, and cautions
- Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methodsComplete the table below. For the function, identify the reagent if it is a stain, clearing agent, mountant, fixative, dehydrant, embedding medium, or adhesive. Then give 2 common health hazards of each. Reagent Function Health hazards 1. FAA 2. Ethyl alcohol 3. Xylene 4. Acetone 5. 10% formalin 6. paraffin 7. Harris Hematoxylin 8. Mayer's albumin 9. Canada balsam 10. Distilled waterWhat is the physical appearance (solid, liquid, semi-solid) of following media: agar plate
- list the stains and decolorizing agent used in acid fast staining method.Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid Schiff technique 2. Grocott's hexamine silver technique 3. Ziehl-Neelsen stain 4. Lactophenol blue stain 5. Mehtamine silver technique 6. Shikata's orcein method 7. Mann's methyl blue-eosin stainStep-by-step smear preparation of Grams stain
- Write down the materials, reagents, Principle, procedure and results for Mann’s methyl blue-eosin stainChoose the 4 most correct steps from the list below for a simple positive stain and organize them into the most correct order. Briefly explain the purpose of each step you chose. • Heat fix • Dip slide in acid alcohol • Wash slide with ethanol • Apply Congo Red to the slide • Apply Crystal Violet to the slide • Make a smear • Wash slide with waterExplain the importance of steam during the acid-fast staining procedure. Discuss the advantages of differential staining procedures over the simple staining techniques. List and briefly describe the functions/purpose of each of the different stains used in acid fast staining.