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- Step-by-step smear preparation of Kinyoun MethodIn four steps, describe the simple staining procedure.INSTRUCTION: Answer the question properly Do not copy in Google, plagiarize checker will be used. QUESTION: In the preparation of Bacterial smear and Gram Staining, what is the purpose of: a) heat-fixing the smear b) prolonged application of heat (without breaking slide) on the smear?
- Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?Identify that chemical mordant used in the cold method of Acid-fast staining.Cause-and-Effect Analysis: Given the conditions below, describe the effect on the staining procedure and explain briefly: 1. Excessive heat was applied during fixation 2. Low concentration of crystal violet used during gram staining 3. Excessive washing between steps 4. Insufficient decolorization 5. Excessive counterstaining
- In preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?Why is it essential that smears be air-dried? Why can’t they be gently heated over a flame to speed up the drying process?Protocol for Mann's methyl blue eosin stain technique
- What is Ziehl Neelsen bacterial staining technique, and what is the target organisms and the purpose of its application? LONG ANSWER NEEDED, DETAILED. Thank you i’ll rate.Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?What are the principle and basic concepts of SMEAR PREPARATION? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)