Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining? 2. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method. 3. What is the specific diagnostic value of this staining procedure?
Q: Explain the reasons you would use a negative stain?
A: In a negative stain the slide is actually stained. This is because the negatively charged stain…
Q: You receive a bacterial culture in the lab and your job is to figure out information which will be…
A: Best option to stain a bacterial culture is Gram Stain Method. It is used to determine gram status…
Q: Why must heat or a surface-active agent be used with application of the primary stain during…
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin
A: The living cells can be seen under a microscope. The cells is not visible properly as it is…
Q: 3. Draw the specimen observed under endospore staining 4. Draw the specimen observed under…
A: Endospore staining: a. It is a differential stain used to distinguish between the vegetative cells…
Q: What are the principle and basic concepts of DIFFERENTIAL staining?
A: The simple dye is used in the technique of simple staining to highlight only the specific structures…
Q: In four steps, describe the simple staining procedure.
A: A stain is used to clearly view a specimen under the microscope. In simple staining, bacteria are…
Q: Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methods
A: Ziehl neelsen staining principle The presence of mycolic acids in the cell walls of acid-fast…
Q: Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can…
A: According to the guidelines we have to answer first 2-3 question rest you can ask separately thank…
Q: What are the principle and basic concepts of NEGATIVE staining?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: Notes on Mann's methyl -blue eosin stain
A: Staining is a technique that is used in microscopy to visualize the cells and cell components in a…
Q: Select all of the following that represent examples of positive stains. A) nigrosin B) methylene…
A: Staining is a technique usually employed to enhance the contrast of specimens in microscopy. Many…
Q: Why should a young culture be used in gram staining rather than an old culture? b. Why is it…
A: To answer this question first, we need to understand what is gram staining and what are the steps of…
Q: What are the most commonly used methods in staining? why?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a tiny scale.…
Q: Describe how simple staining and fluorescence staining are similar and how they are different. What…
A: Microscopy is a technical field for the use of microscopes to visualize objects and locations of…
Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…
Q: .Leifson's stain is made up of tannic acid, basic fuschin stain prepared in alcohol base .A .B F .c…
A: Leifson's stain is a method used to identify the infections of the Helicobacter pylori bacterium.…
Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct) a…
A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…
Q: If you conducted an acid-fast stain on a sputum sample from a person with tuberculosis, what would…
A: The acid-fast staining technique is a differential staining technique. The bacteria which get…
Q: 1. What is the usual stain used for staining a sputum smear? 2. Give the principle of acid fast…
A: Microorganisms are microscopic organisms that are not visible to naked eyes. Several microorganisms…
Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used
A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…
Q: 1. Define Differential Staining. 2. Application of differential staining technique.
A: "Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Types of Differential Staining techniques. 1. Gram stain 2. Acid-fast stain Please…
A: Staining is used in microscopy to highlight the structures to be viewed under microscope. It is of…
Q: What are the principle and basic concepts of Simple staining
A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…
Q: a. Why must heat be used with the application of the primary stain during endospore staining?
A: Defination- An endospore is a non -reproductive structure which is dormant and tough produced by…
Q: What is the secondary stain in both acid-fast stain methods?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: What is the usual stain used for staining a sputum smear?
A: Since you have asked multiple questions, we will answer only first question for you. In order to get…
Q: write the protocol for mann's methyl blue-eosin staining, including their reagents, materials,…
A: Reagents: Alkaline alcohol reagent –100ml of absolute Ethanol or Methanol containing 0.2ml 0.1N…
Q: Use the table to differentiate between agarose gel electrophoresis and SDS-PAGE based on the…
A: Agarose Gel Electrophoresis is the technique of separating DNA or protein. SDS-PAGE is the technique…
Q: name 5 things about method staining
A: INTRODUCTION Staining Staining is a method that is used in medical field and histology to see things…
Q: 1. What is the purpose for using stains? What microbial characteristics can one ascertain from a…
A: NOTE: AS PER OUR GUIDELINES WE ARE ONLY ALLOWED TO ANSWER FIRST QUESTION. PLEASE RESUBMIT THE OTHER…
Q: The acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C.…
A: Acid fast bacteria are gram positive bacteria . They are unique among prokaryotes and normal gram…
Q: You can perform a specific staining method to confirm the purity on the culture. Describe this…
A: Staining methods are used to differentiate between two bacteria depends on their cell wall. To…
Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…
A: The staining is done to enhance the visualization of cell and certain cellular component under a…
Q: 6. What is the function of the iodine solution in the Gram stain? If it were omitted, how would…
A: Gram stain is a technique used for staining bacteria in tissues.
Q: The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C.…
A: To differentiate between the vegetative cells and the endospores, a differential staining technique…
Q: what effect would failing to drain the smear in-between staining have on your judgment of the gram…
A: Gram staining is a technique used to differentiate between two groups of bacteria namely…
Q: Explain acid fast staining?
A: Introduction There are several methods to differentiate bacteria into different groups, staining…
Q: 1-What microbial characteristics can one ascertain from a simplest stain? 2-A student is directed…
A: Gram staining method: This technique is useful in identifying two groups of bacteria based on the…
Q: Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid…
A: (AS PER OUR GUIDELINES WE ARE PROVIDING YOU ANSWER OF 1ST TWO QUESTIONS. YOU CAN POST OTHER…
Q: What is the purpose for using stains? What microbial characteristics can one ascertain from a simple…
A: Microorganisms are the tiny organism that cannot be visualised by naked eyes therefore microscopy is…
Q: Which of the following is/are true regarding the acid-fast stain? (There may be more than one…
A: Answer First option Non-acid-fast microbes appear blue in a completed acid-fast stain.
Q: In staining, why is there a need for contrasting stains?
A: Answer
Q: 2. Give the purpose of each of the following reagents in a differential staining procedure: (Primary…
A: Differential Staining uses multiple chemical stains to view the microscopic organisms. It is because…
Q: k there are 2 staining reagents applied in differential s
A: Differential Staining- It is a staining process which uses more than one staining reagent. it is…
Q: Give the three disadvantages of using irradiation.
A: Irradiation is a comparatively newer technology of food preservation in which food item is…
Q: In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the…
A:
Q: Explain the importance of steam during the acid-fast staining procedure. Discuss the advantages of…
A: Some microbes and their structure are very difficult to be observed under the microscope . So , for…
Q: How can the thickness of the smear affect the staining procedure?
A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…
Q: ) Outline or discuss what happens to a gram negative and gram positive cell at EACH Individual step…
A: On the basis of lipid content and peptidoglycan layer in the cell wall, bacterias are classified…
1. Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?
2. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method.
3. What is the specific diagnostic value of this staining procedure?
4. What is the specific diagnostic value of this staining procedure?
5. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method.
6. What is the specific diagnostic value of acid fast staining procedure?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- 2. Give the purpose of each of the following reagents in a differential staining procedure: (Primary stain, Counter stain, Decolorizing agent and Mordant)Explain the importance of steam during the acid-fast staining procedure. Discuss the advantages of differential staining procedures over the simple staining techniques. List and briefly describe the functions/purpose of each of the different stains used in acid fast staining.1. What is the usual stain used for staining a sputum smear? 2. Give the principle of acid fast staining and gram staining. 3. Explain the Bethesda System. 4. How will you instruct a patient that will undergo a brochoscopy?
- 1. Define Differential Staining. 2. Application of differential staining technique.1.) What is the purpose of acid-fast staining? What information can you gain from this procedure? Please explain.Choose the 4 most correct steps from the list below for a simple positive stain and organize them into the most correct order. Briefly explain the purpose of each step you chose. • Heat fix • Dip slide in acid alcohol • Wash slide with ethanol • Apply Congo Red to the slide • Apply Crystal Violet to the slide • Make a smear • Wash slide with water
- Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can confirm whether a bacterial smear has been correctly prepared or not. Why should you be careful not to underheat a smear during the heat-fixing process? Why do you think the presence of grease or dirt on a glass slide will result in a poor smear preparation? Cite two or three reasons.1.)What is the purpose of a counterstain? 2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant? 3. True or False? The oil objective should make contact with the oil on the slide. 4. Why is it necessary to let bacterial smears completely air dry before heat fixing? 5.Why should controls be included wherever possible for any staining technique? 6. Why is it necessary to heat the slides while staining for endospores?What are the principle and basic concepts of Simple staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)
- 1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantage of heat fixing?1. a) What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain?b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.What are the principle and basic concepts of NEGATIVE staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)