Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain
A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…
Q: Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram…
A: Gram staining is the common procedure followed to distinguish the bacteria based on their cell wall.…
Q: is it essential that the primary stain and counterstain be of contrasting colors
A: Gram staining is one of the crucial type of differential straining .It divides bacteria into two…
Q: Distinguish between simple, differential, and structural stains,including their applications.
A: Staining is a method performed at a microscopic level to enhance the color in the sample. There are…
Q: Why is heat necessary in spore staining?
A: BASIC INFORMATION SPORULATION It is a process during which spores are formed. We can find it in…
Q: Principle and procedure for Mann’s methyl blue-eosin stain
A: Methyl blue stain Methylthioninium chloride or methyl blue is a stain use to highlight animal…
Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?
A: bacteria like Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…
Q: What color would gram positive cells appear if ghr safranin staining step was left out of staining…
A: Gram staining is a technique used for differentiating the bacteria based on their cell wall…
Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…
A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…
Q: Differentiate between negative and positive staining, givingexamples.
A: These are the staining methods that are used to see bacteria under a microscope.
Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…
A: Simple staining: Simple stain uses only one stain for staining bacterial cells. It gives basic…
Q: What is a differential stain? why is it used?
A: Differential stain is a type of stain use to identify organisms.
Q: Give the principle of acid fast staining and gram staining.
A: Microorganisms are extremely small in size and hence cannot be visualized directly. Specialized…
Q: What is the principle of Giemsa staining and components of Giemsa stain?
A: The cells are the basic structural and fundamental unit of the living system. It consists of…
Q: What is the difference between simple stains anddifferential stains?
A: Simple stains : It generally make all of the organisms in a sample appear to be the same color even…
Q: Defi ne dyes and describe the basic chemistry behind the process of staining.
A: Staining is a method by which contrast in a sample is enhanced usually at the microscopic level.…
Q: Describe how simple staining and fluorescence staining are similar and how they are different. What…
A: Microscopy is a technical field for the use of microscopes to visualize objects and locations of…
Q: What are the Types of Differential Stains? explain with an example.
A: Bacteria are prokaryotes that are devoid of any membrane-bound organelles. They are differentiated…
Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used
A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…
Q: explain the spotting effect of ethyl alcohol, acetone and coconut oil when place into the paper
A: When ethyl alcohol is spotted onto paper, it wets the paper and after some time this spot will…
Q: Stains Primary Stain Mordant Destain Secondary Stain Diagram of Stain Simple Stain N/A N/A N/A Gram…
A: This question is regarding different staining techniques of microorganisms.
Q: Protocol for Mann's methyl blue eosin stain technique
A: The anterior pituitary gland and viral appearance are stained with a polychromatic solution of…
Q: write the protocol for mann's methyl blue-eosin staining, including their reagents, materials,…
A: Reagents: Alkaline alcohol reagent –100ml of absolute Ethanol or Methanol containing 0.2ml 0.1N…
Q: What is safe view dye for? Compare it with Ethidium Bromide.
A: Safe view dye is a nucleic acid stain which is used for the detection of double-stranded DNA,…
Q: Differentiate the “hot method” and “cold method” of acid-fast staining
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: Differential stains function to
A: The widely used staining method in the microbiology is the differential staining that are gram…
Q: Give the color reaction in Wright’s stain and the function of these
A: Wright's stain is a polychromatic stain consisting of a mixture of eosin and methylene Blue. It is a…
Q: Differentiate between simple and differential stains
A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question…
Q: Select all of the following that represent examples of positive stains. Check All That Apply…
A: Staining is the common laboratory procedure which can be specific and general according to the use.…
Q: Why is it necessary to use contrasting stains while staining
A: Introduction:- Cell staining is a technique that allows you to see cells and cell components more…
Q: Positive Staining Negative staining
A: Staining Procedure Dye Charge Of The Chromophore/ Colored Ion Charge Of The Target Cellular…
Q: The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C.…
A: To differentiate between the vegetative cells and the endospores, a differential staining technique…
Q: What is a crystal violet stain and its purpose?
A: Crystal violet is that the name given to the cluster of similar organic compounds that are used as…
Q: Outline the main differential stains and how they are used.
A: The detection and observation of cells and microorganisms in their natural state under microscope is…
Q: Define dyes and describe the basic chemistry behind the processof staining.
A: Detection and observation of cells and microorganisms in their natural state under microscope is…
Q: Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid…
A: (AS PER OUR GUIDELINES WE ARE PROVIDING YOU ANSWER OF 1ST TWO QUESTIONS. YOU CAN POST OTHER…
Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…
A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…
Q: When performing a negative stain, how does it work and why does it only stain the background?
A: * Negative stain is practice of applying heavy metal salt stain to sample for observation hence…
Q: In staining, why is there a need for contrasting stains?
A: Answer
Q: How is staining techniques useful in the clinical settings
A: Staining: A sample at the microscopic level can easily be visualized and distinguished from the…
Q: For What purpose is Giemsa stain used for?
A: Staining is a technique used in microscopy. Staining is mainly used in biology to highlight the…
Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
Q: Briefly describe the uses of Special stains.
A: Routine stain: It refers to the hematoxylin and eosin (H&E) staining that is used routinely to…
Q: Name two fixatives used in staining?
A: Histology is the study of ultrastructure of tissues using thin sectioning techniques like microtomy,…
Q: What is the difference between simple and differential stains? Give an example of a differential…
A: Bacteria are microorganisms that are colorless for most of the part and in order to study their…
Q: Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation…
A: Flagella is a hair-like structure that is found in bacteria, archaea, and Eukaryota. It allows the…
Explain the mechanism of staining for each of the stains used.
- Iodine
- Methyl green
- Nigrosin
Step by step
Solved in 3 steps
- Select all of the following that represent examples of positive stains. Check All That Apply nigrosin methylene blue crystal violet India ink safraninFor each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain Cationic or Anionic Dye malachite green crystal violet nigrosin safraninIdentify that chemical mordant used in the cold method of Acid-fast staining.
- Write down the materials, reagents, Principle, procedure and results for Mann’s methyl blue-eosin stainCreate paragraphs to differentiate among the following terms: positive stain, negative stain, simple stain, and differential stain. please provide minimum of 400 wordsWrite down the materials, reagents, Principle, procedure and results for Ziehl-Neelsen stain
- Give the procedure of Sabin-Feldman dye test. Describe the positive and negative results.Explain the importance of steam during the acid-fast staining procedure. Discuss the advantages of differential staining procedures over the simple staining techniques. List and briefly describe the functions/purpose of each of the different stains used in acid fast staining.Is it possible to perform double or triple staining using fluorescent dyes? Explain.
- Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methodsWhat are the principle and basic concepts of Simple staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle, procedure and results.