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- How does DNA replication occur in a precise manner to ensure that identical genetic information is put into the new chromatid? See Figures 8.12 and 8.13. FIGURE 8.12 In DNA replication, the two polynucleotide strands uncoil, and each is a template for synthesizing a new strand. A replicated DNA molecule contains one new strand and one old strand. This mechanism is called semiconservative replication. FIGURE 8.13 A close-up look at the process of DNA replication. (a) As the strands uncoil, bases are added to the newly synthesized strand by complementary base pairing with bases in the template strand. The new bases are linked together by DNA polymerase. (b) DNA synthesis can proceed only in the 5 3 direction; newly synthesized DNA on one template strand is made in short segments and linked together by the enzyme DNA ligase.What is the function of DNA polymerase? a. It degrades DNA in cells. b. It adds RNA nucleotides to a new strand. c. It coils DNA around histones to form chromosomes. d. It adds DNA nucleotides to a replicating strand. e. None of these.Why is DNA replication called semiconservative?
- Which protein is needed to lay down a segment of RNA complementary to the DNA before replication can begin? O RNA pelymerase NI O primase O helicase O DNA polymerase lII O topoisomeraseWhich one is required for elongation step of DNA replication on the lagging strand BUT NOT in leading strand? deoxyribonucleotides O DNA topoisomerase O DNA polymerase O single-strand binding protein O DNA ligaseIf a cell has trouble unwinding the DNA and relieving strain far ahead of the replication fork, in which of the following genes is there a mutation? Beve reverse transcriptase O gyrases/topoisomerases O single stranded binding proteins O ligase O helicase
- What role do the following enzymes play in DNA replication? DNA polymerase Helicase DNA ligaseIf a mutation occurred that prevented single stranded binding proteins from binding, the result would be: O Replication would not be initiated O DNA would be synthesized on the leading strand, but not the lagging strand Replicating would be initiated, DNA would unwind, RNA primers would be laid down, but DNA polymerase would not bind O The newly synthesized RNA would not be able to leave the DNA template Replication would be initiated, but RNA primers would not anneal to the DNAWhich of the following enzymes has the fastest rate of replication in E. coli DNA Replication? O They all replicate at the same speed DNA Polymerase l O DNA polymerase delta O DNA polymerase alpha O DNA Polymerase III
- DNA polymerases are processive, which means that they remain tightly associated with the template strand while moving rapidly and adding nucleotides to the growing daughter stand. Which piece of the replication machinery accounts for this characteristic? Helicase Sliding Clamp Single Stranded Binding Protein PrimaseWhich of the following is correct about the function of DNA Ligase? DNA ligase is used on the leading strand and it joins any disconnected strand together O DNA ligase is used on the lagging strand which helps in joining fragments of the strand together O DNA ligase is used on the lagging strand and it aids in correcting mistakes on this specific strand O DNA ligase is used to maintain the replication fork structureConsidening the structure of DNA, what kind of bonds hold one nucleotide to another within one side of the DNA molecule? O hydrogen O phosophodiester O lonic peptide