What relates to post-transcriptional modifications Polyadenylation Phosphorylation Acetylation 5'-capping 3'-capping Methylation ADP-ribosylation Splicing Alternative splicing
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- Substrate Binding by RNA Polymerase RNA polymerase has t binding sites for ribonucleoside triphosphates: the initiation site and the elongation site. The initiation site has a greater Km for NTPs than the elongation site. Suggest what possible significance this fact might have for the control of transcription in cells.A normal mRNA that reads 5’ - UGCCAUGGUAAUAACACAUGAGGCCUGAAC- 3’ has an insertion mutation that changes the sequence to 5' -UGCCAUGGUUAAUAACACAUGAGGCCUGAAC- 3’. Translate the original mRNA and the mutated mRNA, and explain how insertion mutations can have dramatic effects on proteins. (Hint: Be sure to find the initiation site.)All about splicing A. snRNPs interactions will bring the 5' and 3' splice sites together in the pre-mRNA B. lariat formation is necessary to bring the branch site with the 5' splice site of the intron C. an unstable 5'P and 2'OH phosphodiester bond helps form the lariat D. exon 5' splice site consensus sequence GU and its 3' splice site AG are recognized by snRNPs E. various types of snRNPs and the pre-RNA come together to form the spliceosome A, B, C, E only A, B, C, D, E A, B, C only B, C, D, E only
- Help me figure out how I should interpret and read these graphs. We are looking to see whether the m6a acceptor mutant will have an impact on splicing. Just need a detail breakdown thanks. I am presenting at journal club and need help interpreting will the goals in mind. We are currently conducting experiments to understand how the splicing of circE7 relates to the splicing of linear E6*I. Goal is to determine whether the mutation impact splicing. Trying to understand whether m6A will impact splicing. Sm should impact splicing ratio. My PI stated that the results show that it inhibited linear splicing and promoted backsplicing. I need a detailed explanation for the entirety so I can understand. Why is a nonsense suppressor tRNATyr, even though ithas a mutant anticodon that cannot recognize a tyrosinecodon, charged with tyrosine by Tyr tRNA synthetase?GACA CAAT box A site P site E site DREASAARE III III III III III factors and RNA pol to bind to in order to activate gene expression. Accepts charged tRNA molecules whose anticodon complements the codons of mRNA. Holds a nascent elongating polypeptide before final release. Unique sequence found in bacteria between TATAAT and START. 5 upstream consensus motif that is easily melted for transcription purposes in prokaryotes.
- 19Eukaryotic messenger RNA and hnRNA both have poly (A) tails at their 3'-ends. Yesorno 20Messenger RNA splicing and export are uncoupled processes. Yesorno twenty oneWhich of the following plays an important role in directing newly synthesized proteins to specific cellular destinations?A.signal sequenceb.nuclear localization signalc.chain elongation stretchD.Shine – DalgarnoSARS E You have generated several random mutations in this region. One of them is - 5'-AAUUACCUAUAGAUUGUUU-3' What may be the mutant protein sequence Enter the single letter code for the amino acids. For a stop codon (if any) enter: STOP And fill subsequent blanks with: N/A For example: if you the sequence you need to enter is: M*, enter M N/A N/A N/A N/APost-transcriptional modifications in eukaryotic RNAS A. addition of CCA at the 5'end of the RNA for binding with amino acids B. removal of introns and possibility of alternative splicing in joining different exons C. addition of guanyly residue from GTP to nascent MRNA at the 5' end D. Ten (10) Adenine nucleotides are added to the 3'end of the nascent MRNA E. some bases are modified in tRNA and rRNA (A) А, В, С, Е only в) А, В, С, D only C) A, B, C, D, E D B, C, E only
- Post-transcriptional modifications in eukaryotic RNAS A. addition of CCA at the 5'end of the tRNA for binding with amino acids B. removal of introns and possibility of alternative splicing in joining different exons C. addition of guanyly residue from GTP to nascent mRNA at the 5' end D. Ten (10) Adenine nucleotides are added to the 3'end of the nascent MRNA E. some bases are modified in tRNA and rRNA A A, B, C, E only B, C, E only (c) A, B, C, D, E A, B, C, D onlyGTTTTCACTGGCGAGCGTCATCTTCCTACT 8. What is the function (e.g. transcriptional regulation, transmembrane signaling, kinase, protease, etc.) of the protein(s) encoded by the gene.Match the following (Most appropriate combinations only): IF1 IF3 IRES Shine-Dalgarno sequence m7-G Cap aminoacyl tRNA synthetase proofreading elF4E [Choose ] Binds ribosome A site Translation initiation in eukaryotes Translation initiation in Bacteria binds mRNA cap in eukaryotes Binds ribosome E site Correct charging of a tRNA with amino acid Cap-independent translation initiation of some viral mRNAs [Choose ] [Choose ] [Choose ] [Choose ]
