Q: Which of the following results in green output in spectrophotometry experiment? a. Violet light b.…
A: Spectrophotometry: it is used for quantitative analysis of spectra of various wavelengths to compare…
Q: Why is it important to use standards or to develop a standard curve in spectrophotometry?
A: Spectrophotometry is the analytical technique by which the character of a substance is measured…
Q: p between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.
A: A method to measure how much a chemical substance absorbs light by measuring the intensity of light…
Q: What is the diameter of field of view for the high-power objective in millimeters and micrometers?
A: A microscope is a device that allows the examining of small objects, including cells. The picture of…
Q: How does a contaminant such as salt affect the absorption of light by a spectrophotometric sample?
A: Spectrophotometry is a method in which the absorption or transmission properties of a material is…
Q: What total magnification will be achieved if the 10× eyepiece and the 100× objective are…
A: The microscope is an instrument which used to view and observe tiny organism or an object that…
Q: What is the diameter of the scanning field of view in millimeters? ___________
A: The microscope is an instrument which used to view and observe tiny organism or an object that…
Q: WHAT ARE THE Steps in spectrophotometer
A: -Spectrophotometer is an optical instrument which is used to measure the concentration of a known…
Q: What are the differences between scanner, low power objective, high power objective and oil…
A:
Q: Total Magnification is obtained by Select one: A. Magnifying power of both the objective lens and…
A: Magnification is the term used for the increase in the size of the sample when seen under a…
Q: What is shown? Why did we use the slide? What is the arrow pointing to? What did we use it for? What…
A: Microscope is a instrument which is used to visualise the specimen that can't be seen by naked eyes…
Q: If a circular object or specimen extends halfway across the scanning field, what isits diameter in…
A: Field of view is defined as the area of the slide that is seen when an observer looks through a…
Q: what is the importance of getting rf value in chromotography tools?
A: Chromatography: Chromatography is an analytical technique in which compounds in a mixture are…
Q: What are 2 advantages to using absorbance values (from a Spectrophotometer) over a spread plate and…
A: Introduction: Spectrophotometry is a method utilized for the evaluation and portrayal of drab mixes…
Q: What are the light sources used in UV-vis spectrophotometry.
A: In case of visible spectrophotometers, the wavelengths measured are in the visible light range…
Q: Why is it necessary to use immersion oil when the 100X objective is used'
A: Note - we answer one question at a time. Immersion oil is transparent and has specific viscosity and…
Q: What is the relationship between the working distance of an objective lens and its magnification…
A: Microscope is an optical instrument that uses a lens or an arrangement of lenses to magnify an…
Q: What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective…
A: The correct ans is 450X. The total magnification of specimen viewed with a 10x ocular lens and 45x…
Q: If the total magnification is 2000X with the use of a 10X ocular lens, what is the magnification of…
A: Answer- 200X
Q: what is the total magnification if the objective lens is 4x and the ocular lens is 10x?
A: Step 1 Microscopes are instruments that are used for observing small objects and finer internal…
Q: An object is 20µm in diameter. If viewed through a 10X ocular lens and a 40X objective lens, how…
A: Answer: MICROSCOPY : It is a technique for making small things to visible by magnifying through…
Q: What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x…
A: Given: Objective lens 1 magnification= 1000x DFV1 = ? Objective lens 2 magnification= 400x DFV2 =…
Q: the figure shows the result of the spectral analyzer for paper spectrophotometer without any…
A: Paper spectrophotometry is used in printing industry. In the printing industry, a spectrophotometer…
Q: What is the rule for determining total magnification? A. Ocular lens magnification + objective lens…
A: Microscopy is a technique of incorporating microscopes to enlarge the size of objects (invisible to…
Q: Here are the materials and method for the basic spectrophotometer experiment Materials: 1. Paper…
A: A spectrophotometer is a device used to measure the spectrum of visible light. The wavelength and…
Q: What is the total magnification possible with a 10x objective?
A: The microscope is used to view the objects that are not seen through the naked eye. Microscopes use…
Q: Why does one see methyl orange as orange in color? What blank would be used to standardize this…
A: 1. Methyl orange is a pH indicator frequently used in titration because of its clear and distinct…
Q: How can you improve contrast when viewing a specimen?. What should you do if you have difficulty…
A: Step 1 Microscope (Greek. mikros− small, skipein− to see) is an instrument that is used for…
Q: Define each of the following terms: A) What is resolution and how is resolution related to the…
A: According to the question, we have to provide the solution of the questions one by one. These…
Q: What is the main application of Atomic absorption spectrophotometer (AAS)?
A: The Atomic Absorption Spectrophotometer (AAS) is primarily used to quantify the presence of several…
Q: What are the functions of the Fine Focus, Coarse Focus, Stage Clip, Rack Stop, Arm, Eyepiece,…
A: A microscope is considered a laboratory instrument to observe microorganisms, small objects, cells,…
Q: What is the total magnification of 4x objective lens and diameter of field of view in micrometers?
A: Magnification: The amplifying power or we can say, degree to which the object is seen or viewed…
Q: What solution goes into the cuvette when you’re zeroing the spectrophotometer?
A: Spectrophotometer is a device that is used to calculate the absorbance of the light passing through…
Q: If the total magnification of a slide is 400x and the ocular lenses are 10x, what is the magnifying…
A: Magnification is the process of enlarging the apparent size. This enlargement is quantified by a…
Q: Do the calibration factor computed varies according to different magnification (objective lenses…
A: By simply accounting for the inaccuracy, the Correction Factor allows us to attain more accurate…
Q: spectrophotometry, what is the role of the green background in the set-up? What does it represent in…
A: Introduction:- Spectrophotometry is a technique for determining how much light a chemical substance…
Q: what objective lens is the oil objective lens?
A: We have to determine the objective lens that is used for oil immersion.
Q: If the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x,…
A: According to the question, the total magnification of a microscope is 700X.…
Q: is the reason for using blind tube in spectrophotometric and calorimetric studie
A: A method which is used to measure how much a chemical substance absorbs light by measuring the…
Q: What information can be obtained from the slides prepared by the wet mount technique and the hanging…
A: Introduction : The wet mount and hanging mount techniques are used to observe living…
Q: Question 5 What is the total magnification of a sample with an ocular lens power of 15X and using a…
A: A compound microscope has two lens one is ocular or eyepiece lens and objective lens. Objective lens…
Q: What is the differences between ocular magnification and objective magnification?
A: Introduction A microscope's objective lens is the one near the sample at the bottom. It's an…
Q: What are the significance of UV/Vis spectrophotometer in the experiment complexation.
A: UV/VIS spectrophotometer is the technique used to determine the optical properties such as…
Q: How do you know the range in microliters within which a given micropipette can accurately measure?
A: A micropipette is a device used to transfer small volume of samples . It is a vital part in…
Q: A michroscope has a 20x ocular and two objectives of 10x and 43x respectively: calculate the…
A: Apparent magnification of the objective is provided by the proportion of the viewing angle of its…
Q: What is the stationary and mobile phase of spectrophotometry?
A: Introduction A biotechnique based on the principle of quantitative measurement of light intensity…
Q: What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?
A: Spectroscopy is a procedure utilized for the evaluation, portrayal, and basic investigation of a…
Q: If the specimen measures 150mm and you draw it 50mm, how many times you have it reduced?
A: Magnification is defined as the ability to make smaller object to see it in larger manner. In this…
Q: what is experimental error that may occur when reading spectrophotmeter
A: Spectrophotometry is a technique for determining how much light a chemical substance receives by…
What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?
Step by step
Solved in 2 steps
- What is the stationary and mobile phase of spectrophotometry?The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?In spectrophotometry, what is the role of the green background in the set-up? What does it represent in the actual spectrophotometer?
- It is possible to make a perfectly precise measurement?Explain.Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's LawIdentify three factors that would make the reading of a co-oximeter unreliable.
- What is the meaning of the different values given engraved on each objective?What solution goes into the cuvette when you’re zeroing the spectrophotometer?What is the specific objective lens that is used to locate the specimen and first focus? Explain. What is the difference between using a coarse adjustment knob and a fine adjustment knob?