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How does a contaminant such as salt affect the absorption of light by a spectrophotometric sample?
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- In spectrophotometry, what is the role of the green background in the set-up? What does it represent in the actual spectrophotometer?The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?
- Why is it important that the standard curve you create in biological analyses with spectrophotometry is measured using specialty cuvettes?Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement or during the transfer to the spectrophotometer?Which of the following is/are source/s of error in performing a spectrophotometric method? Answer all that apply. Sample is turbid when the absorbance is read. The absorbances were read at maximum absorption. Standard solutions are prepared accurately. The cuvettes have fingerprints.