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What is the impact of new km value 0.1mM compared to previous km value 0.05mM on affinity of the enzyme for substrate?

What is the impact of new km value 0.1mM compared to previous km value 0.05mM on affinity of the enzyme for substrate?

Essentials Health Info Management Principles/Practices
Essentials Health Info Management Principles/Practices
4th Edition
ISBN:9780357191651
Author:Bowie
Publisher:Bowie
Chapter10: Introduction To Coding And Reimbursement
Section10.1: Clinical Classification Systems
Problem 5E
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What is the impact of new km value 0.1mM compared to previous km value 0.05mM on affinity of the enzyme for substrate?
Michaelis Menten Plot.als (Compatibility Model - Microso Home Insert Page Layout Formulas Data Review Acrobat * Cut a Copy Format Painter Calibri 11 AA Wrap Text General BIU - A FI ER Merge & Center S% A Conditional Form Formatting- as Tabi Cipboard Fent Agnment C20 K L M C D Calc. Vo A F. G H 1 (Ethanol) Vo delta 2 0.007 0.06 0.020242 0.001581 035 3 0.015 0.11 0.040357 0.00485 4 0.031 0.16 0.073219 0.007531 03 0.068 0.21 0.125236 0.007185 025 6 0.1 0.23 0.154704 0.00567 0.2 0.28 0.206272 0.005436 0.2 0.3 0.29 0.232056 0.003358 0.28 0.247526 0.001055 Calc. Vo 0.4 0.15 10 0.036664 01 11 Km 0.1 12 Vmax 0.309407 0.05 13 14 16 0.1 02 0.3 0.4 0.5 17 18 19 Fig. 9. Michaelis-Menten Plot using a value of 0.1 mM for Km. Notice: 1) that a new set of calculated values in column C has been generated and 2) that by using these new data a new best fit Michaelis-Menten plot (red curve) has been generated. Fig. 9 shows that the rates for the new enzyme-catalyzed reaction (red curve) were even lower than those obtained with a value of Km of 0.05 mM (red curve in Fig. 8).
Transcribed Image Text:Michaelis Menten Plot.als (Compatibility Model - Microso Home Insert Page Layout Formulas Data Review Acrobat * Cut a Copy Format Painter Calibri 11 AA Wrap Text General BIU - A FI ER Merge & Center S% A Conditional Form Formatting- as Tabi Cipboard Fent Agnment C20 K L M C D Calc. Vo A F. G H 1 (Ethanol) Vo delta 2 0.007 0.06 0.020242 0.001581 035 3 0.015 0.11 0.040357 0.00485 4 0.031 0.16 0.073219 0.007531 03 0.068 0.21 0.125236 0.007185 025 6 0.1 0.23 0.154704 0.00567 0.2 0.28 0.206272 0.005436 0.2 0.3 0.29 0.232056 0.003358 0.28 0.247526 0.001055 Calc. Vo 0.4 0.15 10 0.036664 01 11 Km 0.1 12 Vmax 0.309407 0.05 13 14 16 0.1 02 0.3 0.4 0.5 17 18 19 Fig. 9. Michaelis-Menten Plot using a value of 0.1 mM for Km. Notice: 1) that a new set of calculated values in column C has been generated and 2) that by using these new data a new best fit Michaelis-Menten plot (red curve) has been generated. Fig. 9 shows that the rates for the new enzyme-catalyzed reaction (red curve) were even lower than those obtained with a value of Km of 0.05 mM (red curve in Fig. 8).
Your worksheet and graph should look similar to the figure below. Michaelis Menten Plot.ds [Compatibility Mode] - Microsot Home Insert Page Layout Formulas Data Review View Add-Ins Acrobat * Cut Wrap Text General Calibri Copy Conditional Forma Paste BIU- E Merge & Center Format Painter Formatting as Tabl Clipboard Font Alignment Number C16 B G H K M 1 [Ethanol] Vo Calc. Vo delta^2 2. 0.007 0.06 0.037997 0.000484 0.35 0.015 0.11 0.071402 0.00149 4 0.031 0.16 0.118415 0.001729 0.3 5 0.068 0.21 0.178303 0.001005 0.25 0.1 0.23 0.206272 0.000563 0.2 0.28 0.247526 0.001055 0.2 0.3 0.29 0.265206 0.000615 • Vo 0.4 0.28 0.275029 2.47E-05 0.15 Calc. Vo 10 0.006965 0.1 11 Km 0.05 12 Vmax 0.309407 0.05 13 14 16 0.1 0.2 0.3 0.4 0.5 17 18 Fig. 8. Michaelis-Menten Plot using a value of 0.05 mM for Km. lotice: 1) that a new set of calculated values in column C has been generated anc
Transcribed Image Text:Your worksheet and graph should look similar to the figure below. Michaelis Menten Plot.ds [Compatibility Mode] - Microsot Home Insert Page Layout Formulas Data Review View Add-Ins Acrobat * Cut Wrap Text General Calibri Copy Conditional Forma Paste BIU- E Merge & Center Format Painter Formatting as Tabl Clipboard Font Alignment Number C16 B G H K M 1 [Ethanol] Vo Calc. Vo delta^2 2. 0.007 0.06 0.037997 0.000484 0.35 0.015 0.11 0.071402 0.00149 4 0.031 0.16 0.118415 0.001729 0.3 5 0.068 0.21 0.178303 0.001005 0.25 0.1 0.23 0.206272 0.000563 0.2 0.28 0.247526 0.001055 0.2 0.3 0.29 0.265206 0.000615 • Vo 0.4 0.28 0.275029 2.47E-05 0.15 Calc. Vo 10 0.006965 0.1 11 Km 0.05 12 Vmax 0.309407 0.05 13 14 16 0.1 0.2 0.3 0.4 0.5 17 18 Fig. 8. Michaelis-Menten Plot using a value of 0.05 mM for Km. lotice: 1) that a new set of calculated values in column C has been generated anc
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