A. Lineweaver-Burk plot of the enzyme with increasing amounts of substrate in the absence or the presence of the inhibitor is shown below. Graph A : x-intercept = - 0.007, y-intercept = 0.8 Graph B : x-intercept = - 0.012, y-intercept = 0.8 Graph C : x-intercept = - 0.027, y-intercept = 0.8 Graph D : x-intercept = - 0.039, y-intercept = 0.8 Graph A 4 Graph B Graph C Graph D -0,04 -0,02 0,00 0,02 0,04 1/[Substrate] (uM) (i) Which graph indicates an enzymatic reaction without inhibitor? (ii) Which type of inhibitor is it? Briefly explain. (iii) Which graph indicates the highest concentration of inhibitor? 1/Rate (umol/min)

Biochemistry
9th Edition
ISBN:9781319114671
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Chapter1: Biochemistry: An Evolving Science
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A. Lineweaver-Burk plot of the enzyme with increasing amounts of substrate in the absence
or the presence of the inhibitor is shown below.
Graph A : x-intercept
Graph B : x-intercept = - 0.012, y-intercept = 0.8
Graph C : x-intercept = - 0.027, y-intercept = 0.8
Graph D : x-intercept = - 0.039, y-intercept = 0.8
- 0.007, y-intercept = 0.8
Graph A
4
Graph B
Graph C
Graph D
1
-0,04
-0,02
0,00
0,02
0,04
1/[Substrate] (uM)
(i) Which graph indicates an enzymatic reaction without inhibitor?
(ii) Which type of inhibitor is it? Briefly explain.
(iii) Which graph indicates the highest concentration of inhibitor?
(iv) Calculate the Vmax and Km of the graph showing an enzymatic reaction with the lowest
concentration of inhibitor. Show the steps of calculation and unit in your answers.
Keep 2 decimal places in your answers.
1/Rate (umol/min)
Transcribed Image Text:A. Lineweaver-Burk plot of the enzyme with increasing amounts of substrate in the absence or the presence of the inhibitor is shown below. Graph A : x-intercept Graph B : x-intercept = - 0.012, y-intercept = 0.8 Graph C : x-intercept = - 0.027, y-intercept = 0.8 Graph D : x-intercept = - 0.039, y-intercept = 0.8 - 0.007, y-intercept = 0.8 Graph A 4 Graph B Graph C Graph D 1 -0,04 -0,02 0,00 0,02 0,04 1/[Substrate] (uM) (i) Which graph indicates an enzymatic reaction without inhibitor? (ii) Which type of inhibitor is it? Briefly explain. (iii) Which graph indicates the highest concentration of inhibitor? (iv) Calculate the Vmax and Km of the graph showing an enzymatic reaction with the lowest concentration of inhibitor. Show the steps of calculation and unit in your answers. Keep 2 decimal places in your answers. 1/Rate (umol/min)
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