Anita suspects she might have had Covid-19 about four months ago. Currently, she has no symptoms. What tests should be used to find out if that indeed was the case? cell culture Indirect ELISA Direct ELISA real-time qPCR
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- https://youtu.be/w7aIxiZQ60g Multiplexing agglutination https://youtu.be/uWStmyJ5Qc0 This is the multiplexing agglutination. Lab report I don’t really know what to talk about, the data, conclusions and the purpose of this. Need help pleaseHIV testing uses a(n) ________________ELISA. direct indirectAn ELISA test is useful for detecting the presence of antigens but not antibodies in a sample. true or false
- If the test samples are positive for the antigen in the ELISA we are using, what color will you see? Blue Pink Green O OrangeCan you please check and answer the questions that not answered, that is not graded https://www.studocu.com/en-us/ document/university-of-arkansas/microbiology/other/immunology-lab-worksheet-student/7960193/view Questions from ELISA Simulation Introduction Document (posted in Lab Module 8 folder): 1. What term is the word “antigen” derived from? 2. Where are antibodies found in the body? Plasma of the blood 3. In general, what is the cause of an autoimmune disease? 4. What kind of antibodies are present in patients with systemic lupus erythematosus (SLE)? 5. What does ELISA stand for? enzyme-linked immunosorbent assay 6. In general, what is an ELISA test used to detect? to measure the concentration of antibodies or antigens 7. In an indirect ELISA, which antibody is linked to the enzyme? (i.e the primary antibody from the blood serum or the secondary anti-human antibody) Questions from HHMI Virtual Immunology Lab Website Diagnosis…The direct ELISA test requires only one antibody only one antigen both primary and secondary antibody only secondary antibody conjugated with enzyme system
- You are tasked with measuring the quantity of prostate specific antigen (PSA) in a urine sample using an ELISA strategy. Describe and schematically depict how you would perform the test given the following materials: anti-PSA, biotinylated anti-PSA, streptavidin-HRP, HRP substrate kit (HRP = horse radish peroxidase).A home pregnancy test is an example of a _____ ELISA. Direct IndirectThe questions are for covid testing.
- Following is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300 Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial? Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…Place the steps in the correct order to assess your understanding of the indirect ELISA. Indirect USA Enzyme-linked antibody adkued to wel. Well A WellB Rinse wels. Add colorless substrate. Add known antigen Add sorum sample from patent. Color develops if HIV antibodies were present In serum Rinse well to remove unbound antbodies Reset Copyright CMcaraw-Hil Education, Permission required for reproduction or display. < Prev 23 of 44 NextYou counted 40 colonies on a plate in your dilution series. The plate was inoculated with 1.0ml from a 10-7 test tube, what is the OCD?..