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What are the elution techniques usually followed in HPLC?
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- Enumerate the differences between a serological and a volumetric pipette and enumerate the uses, advantages, and limitations of a glass versus a plastic volumetric pipette.Explain the term gradient elution mode in HPLCDifferentiate between isocratic elution and gradient elution. Why is gradient elution sometimes a necessary technique in HPLC? What are the steps involved in gradient elution?
