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- Explain the term gradient elution mode in HPLC Differentiate between gel filtration and gel permeation chromatography.Differentiate between isocratic elution and gradient elution. Why is gradient elution sometimes a necessary technique in HPLC? What are the steps involved in gradient elution?Which of the following solvents is used in normal phase elution in HPLC? a) Ethanol: Water b) Hexane c) Acetonitrile CH;CN d) Methanol
- Enumerate the differences between a serological and a volumetric pipette and enumerate the uses, advantages, and limitations of a glass versus a plastic volumetric pipette.What are the similarities between fluorescence detectors in HPLC and chemiluminescence detectors in HPLC?describe different type of degasser in HPLC system, and why degassing is important in the middle of HPLC operation?