What are other staining methods that can be used for PAGE?
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topic: gel electrophoresis
. What are other staining methods that can be used for PAGE?
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- Describe how you will perform serial dilution. Write and capture your image well.What is gel electrophoresis and why is it important? Write in detail.Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?
- for gel electropertisis, what would be an appropriate caption for the tableDescribe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail and also provide some better suggestions.
- What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?Image 1 shows raw data of gel electrophoresis. Label/annotate image 1 (lanes, ladder sizes, etc). Explain what your seeing in the gel. Use the following information and picture 2 to assist in labelling. The purpose of this gel electrophorsis is to ensure that your GOI, FAP257, is in each BAC. Protocol that was done for Gel electrophoresis: -Place tray with gel into gel box -Fill gel box with IX TAE until the gel is completely submerged -Remove the comb for wells -Load 10ul of the 1kb Gene Ruler ladder (well 1) -Contains DNA ladder, 6X TriTrack DNA, Loading Dye, and Deionized water -Load other wells -Well 2: Control -Well 3: BAC- 15M5 -Well 4: BAC-39K10 -Well 5: BAC-27N17 -Run the gel at 100V for 30 minutes or until the dye front has migrated 2/3 down the gelWhich of the following is true regarding aceto-orcein staining? Question options: Is ineffective for staining as DNA is soluble in acetic acids. Orcein can be extracted from three different species of flower. Staining occurs via hydrophobic bonding and van der Waals attractions. The active dyes in this strain are small, extremely hydrophobic anionic dyes.
- Make a concept map/flow chart for this technique (Cellulose Tape Perianal Swab)What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide? Paragraph BIUc: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.