What are analytes and how can they be used for biochemical recognition?

Q: a 50.00-mL sample containging an analyte gives a signal of 11.5(arbitrary units).  A second 50-mL…

A: Solution The mechanism of analyte concentration in field-enhanced sample injection (FESI) is…

Q: Calculate the following DNA concentrations and determine the purity of the samples: DNA Dilution…

A: Formula used :-  Concentration of double stranded DNA = 50 µg/mL × OD260 × dilution factor.

Q: The concentration of Ba(103)2 in H20 at 25°C (Ksp = 1.75 x 10-9) Ba(103)2 (s) - Ba2+ + 2103 8.32 x…

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Q: Briefly explain how anodic stripping analysis is able to be used for quantifying DNA

A: Cathodic or anodic stripping voltammetry have been used for a highly sensitive determination of…

Q: particular

A: Difference and simmilarity Enzyme linked immunosorbent is a laboratory technique that uses…

Q: Ref ranges Results 3,7 mg/dl 35mg/dl 12.5 ml/min Tests Creatinine Females <1.2 BUN 5-20 Creatinine…

A: A 60-year-old woman with a history of rheumatoid arthritis, smoking of 60 packs/year, chronic…

Q: What are the different technical considerations in performing the serum protein electrophoresis…

A: Answer - The serum protein electrophoresis (SPEP) test measures specific proteins in the blood to…

Q: (b) Protein concentration can also be determined by UV spectrophotometry. (i) Describe the principle…

A: Answer. UV - Visible spectroscopy is an analytical technique used to determine the absorbance of an…

Q: The following are the goal of chemical spearation process, EXCEPT? O identification of the separated…

A: Here we have to choose correct option which is not included in a chemical separation process.…

Q: Find the [FeSCN^2+]eq ε=4.0×10^3 M-1 cm^-1 at 447 nm

A: According to Lambert-Beer Law, “There is a linear relationship between the concentration and the…

Q: absorbancy of 0.310 was obtained. Set up a standard curve, excluding outliers (experimental and…

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Q: 14. If an ink is made by mixing dyes 1,2 and 3, which chromatogram will be produced from the ink?…

A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question…

Q: The image below is a positive ionization mode Electrospray ionization-mass spectrum (ESI-MS)…

A: A multiple choice problem based on mass spectrum, which is to be accomplished.

Q: ou obtained the following raw data when setting up a Biuret standard curve:   BSA (mg/ml)…

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Q: The electrophoresis of a sample containing 2-amino-4-methylpentanoic acid. (pKa of a-COOH = 2.2, pKa…

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Q: A student performed a chromatography experiment to determine the Rf value of an unknown amino acid.…

A: The Rf value ( retention factor) of a compound is defined as the ratio of distance travelled by the…

Q: Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an…

A: Given

Q: For an enzyme reaction followed spectrophotometrically at 340 nm the Vmax was calculated at 0.14…

A: Mass of enzyme in cuvette = 30 ng = 30 X 10-9 g

Q: ase calculate the unknown concentration of the protein D wih an absorbance value of A554 given the…

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Q: & protein in a solution measured in a 96 well plate You have the following information: volume in…

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Q: Which most accurately describes what the diagram below represent? Reflected light Incident light…

A: It's a Schematic diagram of an SPR based genosensor. The incident polarized light is coupled by a…

Q: Explain why the LC-MS-MS method is superior to the peptide mass mapping method.

A: Peptide mass fingerprinting (PMF) is an analytical technique for protein identification in which the…

Q: ▪ True or False: in a TOF analyzer, a fragment of low mass reaches the detector first ▪ In GCMS when…

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Q: The negative ion mode electrospray ionization (ESI) mass spectrum of an oligonucleotide shows peaks…

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Q: ttering technique. If the formulation of the subject sample has included surfactants or salt…

A: The size of small particles in solution or in suspension is determine by the light scattering…

Q: +Protein Mr pI 12,000 28,000 42,000 66,000 (a;) 103,000 (a2) 111,000 А В 8.4 C 7.2 9. E 5 F 4.2…

A: Size exclusion chromatography is an analytical technique that separates the components of a mixture…

Q: 24. N2O5 - 25. LizSO4 - 26. KOH - 27. H;BO; - 28. FeBr3 - 29. Mg3(PO:)2 - 30. PbCrO4 -

A: Here we have to write name of the compounds-

Q: what are advangtage and disadvantage of using an Atomic Force Microscopy and explain each

A: Atomic force microscopy is a technique by which we study the different type of surfaces like…

Q: which of the following chromatographic methods is best used for separating amino acids?

A: Chromatography is the technique that is used for the separation of different components from the…

Q: If the pKa of an acid is 3.7, what is the Kb value of the acid?

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Q: Why does a molecularly imprinted polymer selectively bind a desired analyte?

A: Molecularly imprinted polymer selectively bind a desired analyte has to be explained.

Q: The mass of a protein was determined to be 92.7 kDa using matrix assisted laser…

A: The relation between kDa (Kilodalton) and Kg (Kilograms)   1 kDa = 1.661 × 10-24 kilogram   By using…

Q: QUESTION 1 You obtained the following raw data when setting up a Bluret standard curve BSA (mgim)…

A: Calibration curve between [BSA] versus Absorbance Considering the linear least regression equation,…

Q: What is the correlation between the level of the spectrophotometer's absor- bance readings and the…

A: Given table: pH of buffer solution  Absorbance 3 1.270 5 0.410 7 0.275 9 0.240 11 1.170

Q: Q1: The concentration of yeast t-RNA in an aqueous solution is 10 M. The absorbance is found to be…

A: Specific absorptivity= 0.0209dm3/mol/cm

Q: Use the following chromatogram of standard amino acids: 24 45 16 19 21 12 15 23 13 14 18 10 12 14 16…

A: To determine the retention time of peak 4 from the given chromatogram.

Q: QUESTION 1 You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml)…

A: Given: absorbance of unknown sample = 0.284 To find: Set up a standard curve,  determine the protein…

Q: You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) 0 1 2 3 4 5…

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Q: Write Ksp for Ca3 (PO4)2 salt ... a. [Ca2+]3 [PO4]² b. [Ca2+] [PO43-1² c. [Ca]³ [PO43-]? d. [Ca2+]²…

A: The compound given is Ca3(PO4)2.

Q: You have a mixture of 100 proteins with molecular weights between 30 and 45 kDa. All proteins in the…

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Q: CHM220 Page 3 он m. CH3 n. HC

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Q: 10. C4H,Br 100 80- 68- 42 20- 2922 8L82 1379 602T 1151

A: Degree of unsaturation of C4H9Br is  as    DU = 4-92-12+1=5-102=0NO degree of unsaturation…

Q: (b) Protein concentration can also be determined by UV spectrophotometry. (i) Describe the principle…

A: Answer. UV spectrophotometry or UV-Visible spectroscopy  is an analytical technique used to identify…

Q: what are the advantages of mediator-based amperometric enzyme sensors for glucose compred to…

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Q: Calculate the iodine number of a tissue sample in Table 1 and describe the source of the sample…

A: Iodine number : it is the measurement of the unsaturation in fat and oil. It is the value of iodine…

Q: 1) a)What is affinity chromatography and what is it used for? b) A drug called Bamlanivimab is…

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Q: By using the following mentioned concentrations and their corresponding  absorbance values prepare a…

A: Using Excel we can plot the dependence of absorbance on the concentration of any sample. The use of…

Q: the

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Q: m Rel. Intensity Proposed Fragment 15 14.0 26 4.8 27 4.7 4.2 42 6.7 43 100.0 58 37.0 N3445

A: Mass spectral analysis: The peak at 58 m/z is a molecular ion peak because the next peak has less…