Calculate the following DNA concentrations and determine the purity of the samples: DNA Dilution Dilution A260 Concentration A260/280 Pure Explain Sample factor DNA? 1 1 ul in a total 10/1 = 1.98 1.56 volume of 10 10ul 2 0.5 ul in a 20 2.5 1.82 total volume of 10ul 3 5 ul in a total 0.86 1.95 volume of 25 ul Show your calculations (for samples 1-3 above) below: DNA conc = (A260 reading) x (absorbance factor) x (dilution factor)| If you have 20 ul of each DNA sample above (samples 1-3), what is the total DNA yield (concentration)? Show your calculations below:
Coding Strand of DNA
When pointing to DNA transcription, the coding strand is found to be the DNA strand whose base sequence is indistinguishable from the base sequence of the RNA transcript developed. It is this strand that comprises the codons, while the non-coding strand comprises the anti-codons.
Nucleotide
Both DNA and RNA are composed of organic molecules known as nucleotides. Hence, nucleotides are known as the basic building blocks of nucleic acids. These substances play a role in various processes such as cell signalling, enzyme reactions, metabolism, and so on.
Structure of Cytosine
Cytosine is among the five primary nitrogenous bases of which DNA and RNA and are being used in storage and transportation of genetic makeup within a cell. Adenine, guanine, thymine as well as uracil are the remaining four nucleobases.
Step by step
Solved in 3 steps
