Using the 5 major steps, make or create your own flow diagram of the genetic engineering process (isolation, cutting, ligation, transformation, colony screening)
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- Using the 5 major steps (isolation, cutting, ligation, transformation, colony screening), make your own flow diagram of the genetic engineering process.Remember that although there are many interesting ideas about genetic engineering of plants and animals, this is specifically about GE bacteria. Please be sure you are answering the following questions 3) What are the benefits (or potential benefits) of the engineered bacterium? 4) What are the risks (or potential risks) of the engineered bacterium?Using the 5 major steps, make or create your own flow diagram of the genetic engineering process.
- Insert a specific stress-tolerant gene into a tissue cultured plant batch. What can be your possible approach? You may only give a flow chart to show your process.explain how the following genotypic andphenotypic methods of microbe identification is done. Use diagrams or schematic representations to explain yourwork. 1. Pulsed-Field Gel Electrophoresis (PFGE)2. Whole Genome Sequencing (WGS)What type of experiment would you be most likely to use to demonstrate that protein X was directly phosphorylated by the M-cyclin/CDK pair a flow cytometry experiment a PCR based assay an in vitro assay a fluorescence microscopy experiment
- explain how the following genotypic andphenotypic methods of microbe identification is done. Use diagrams or schematic representations to explain yourwork. (1) Restriction Fragment Length Polymorphism (RFLP)(2) Pulsed-Field Gel Electrophoresis (PFGE)(3) Whole Genome Sequencing (WGS)(4) Gram Staining(5) Biochemical Reactions: Multiple Tube Fermentation Technique(6) Biochemical Reactions: IMViC Testdescribe the blue-white colony selection method in the recombinant colony screening, and present a brief argument for its use in biotechnology labs.Your supervisor asked you to insert a specific stress-tolerant gene into a tissue cultured plant batch. What can be your possible approach? You may only give a flow chart to show your process.
- Imagine you are a cytogeneticist preparing a karyotype, but you forgot to add the Giemsa stain in the middle part of the protocol. You are not aware of the mistake, until you look at the slides, as you are getting ready to photograph the metaphase spreads. What would you see on the slides, that would tell you that you made a mistake earlier in the karyotype protocol?Regarding STR markers used in forensic science. Tick all the correct statements: no correct statement the PCR primers used to amplify STRs are located in the repeat units PCR primers to amplify STRs are located on both sides of the repeat units the PCR primers used to amplify STRs are coupled to a fluorochrome which is essential for the detection of amplicons they are absent from the gonosomes the allelic frequencies of STR markers vary according to the ethnicity of the individuals genotyped they are present homogeneously throughout the nuclear genomeCreate your own flow diagram of the genetic engineering process using the 5 major steps.