Gene therapy: a) what disease is it used for? b) what genetic defect causes the disease? c) what and how viral vector is used AND/OR CRISPR-Cas is used?
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Question:
Gene therapy:
a) what disease is it used for?
b) what genetic defect causes the disease?
c) what and how viral vector is used AND/OR CRISPR-Cas is used?
d) what is in the horizen re. development of new virus-based gene delivery vesicle?
Step by step
Solved in 2 steps
- 15) Why are viruses an ideal candidate for gene delivery in gene therapy? a) What are TWO limitations of viruses as gene delivery vectors in gene therapy?4) List 3 things that a potential vector must have in order to be useful. 5) Sometimes the gene for the enzyme glutathione-S-transferase (GST) is used in purifying your protein of interest. Briefly explain how this is done. 6) What enzyme is used to create CDNA from MRNA?Given that viruses must be cultivated to make vaccines against viral diseases, discuss the advantages and disadvantages that might occur with each approach (embryonated egg versus cell culture) to growing animal viruses.
- Q.No.4. A) Design a oligonucleotide probe for provided gene sequence using all the guidelines for efficient probe designing. ACAACCCCAAGCCTTCAACCACCCCCTTCCCCCAAATTAGAGATCGATCTCAAGAAGAAGAATGGGTTCCGTCTCTCGCTCTTCTTTGGATCAGAAGCTGGCCATGGCAAAGCGCTGCTCCCACGAGGGAGTTGTCGCGGGAGCAAAGGCGGCCGTGGTTGCAACTGTTGCCTCGGCCATTCCTACTTTGGCTAGCGTTAGGATGATCCCATGGGCGAGGTCCTTCCTTAATCCCGCAGCTCAGGCCCTCATCGTTTCATCAGCGGCGGGGGCGGCGTACTTCATAGTTGCGGACAAGACB) Specify the method for Purification of DNA fragment after digestion.(Genetic engineering).What are the major components of the CRISPR-Cas9 system? What mechanism does it employ to combine DNA? Explain the process of how the CRISP-Cas9 system is able to create recombinant DNA. Relate the idea of gene modification to the fields of vaccines and applied microbiology as well.32. The CRISPR-Cas system helps some prokaryotes defend against the phage! Which of the following statements correctly describes how this system works? A) it produces RNA that is translated into proteins that attack viral DNA. B) it inserts specific genes randomly into the prokaryotic genome ) it causes the synthesis of proteins that are then secreted by the prokaryote that attack viruses in the environment. D) it cuts viral DNA at specific sequences before it can replicate.
- Choose two genes from Figure 4.6 and draw a graph to represent the change in transcription over time.3) Would mRNA vaccines be as effective against bacteria as they are against viruses? Why or why not? Questions related to SARS-CoV-2 Inhibitor Science Article (Zhang et al.) 1) What is the function of the viral main protease? 2) What is the amino acid recognition sequence that is cleave by this main protease? Indicate where this protease cuts? 3) Would interfering with this protease effect other proteases in human cells? 4) How closely related are the amino acid sequences between SARS-CoV-2 and SARS-CoV? What does this suggest about focusing research on inhibiting this enzyme?Cloning vectors are not just limited to bacterial plasmids. Bacteriophages and M13 phage vectors are also commonly utilized in the cloning process. State any five (5) key criteria to be an effective cloning vector.
- Briefly and in simple terms, describe how CRISPR could be utilized in treating genetic conditions such as Batten disease.3 types of common coronavirus tests (RT-PCR for viral RNA with Taqman detection, lateral flow antibody tests, and lateral flow antigen tests) have been examined. a) The RT-PCR with Taqman detection is used to test for viral RNA in univeristy laboratory for testing saliva samples. Why do you think a univerisity chose chose this test to screen students/faculty/staff for Covid-19 on a regular basis? Explain your answer by discussing advantages/disadvantages of the test. b)An antigen test was used to screen students in when they arrived on campus. Why do you think a univeristy chose this test for this purpose? What are advantages and disadvantages of this test? c)Do you think the RT-PCR test with Taqman detection for viral RNA is best test for regular screening? Justify your answer.Among the following statements regarding the use of viral vectors, which is (are) true: (can be more then one answer) A.Viral vector transfection has the defect of rapidly killing cells, given the high toxic potential of viruses. B.Generally, the effectiveness of a viral infection transfection is much higher than an electroporation or cationic liposome transfection. C.Even before infecting a cell, a virus can begin to express the indicators, accelerating the process of expression of the indicator in the culture medium. D.Adeno-associated viruses are little used in biophotonics, as they require simultaneous adenovirus infection.