To verify the is indeed inside your plasmid, you'd like to do a colony PCR. But you need primers for your reaction. Which of the following primer pairs would probably work for verifying your insert is actually present in the plasmid? 5' ATGTTTATTTTCTTATTATTTTTTACTCTCACTAGTGGTAGTGACCTTGACCGGTGCACCACTTTTGATG ATGTTCAAGCTCCTAATTACACTCAACATACTTCATCTATGAGGGGGG TTTACTATCCTGATGAAATTTT (Very long, but a bunch of nucleotides her e)...... TCTTGCTTTGTTGCATGACTAGTTGTTGCAGTTGCCTCAAGGGTGCATGCTCTTGTGGTTCTTGCTGCAA GTTTGATGAGGATGACTC TGAGCCAGTTCTCAAGGGTGTCAAATTACATTACACATAA 3' Forward: 5' GGT AGT GAC CTT GAC CGG 3' Tm= 59.8 OA. Reverse: 5' CAA ATT ACA TTA CAC ATA A 3' Tm= 47.4 Forward: 5' ATG TTT ATT TTC TTA TTA TTT 3' Tm= 47.1 C OB. Reverse: 5' TAT GTG TAA TGT AAT TTG ACA CCC 3' Tm= 58.4 Forward: 5' GGT AGT GAC CTT GAC CGG 3' Tm= 59.8 Reverse: 5' GTG TAA TGT AAT TTG ACA CCC TTG 3' Tm: 59.4
To verify the is indeed inside your plasmid, you'd like to do a colony PCR. But you need primers for your reaction. Which of the following primer pairs would probably work for verifying your insert is actually present in the plasmid? 5' ATGTTTATTTTCTTATTATTTTTTACTCTCACTAGTGGTAGTGACCTTGACCGGTGCACCACTTTTGATG ATGTTCAAGCTCCTAATTACACTCAACATACTTCATCTATGAGGGGGG TTTACTATCCTGATGAAATTTT (Very long, but a bunch of nucleotides her e)...... TCTTGCTTTGTTGCATGACTAGTTGTTGCAGTTGCCTCAAGGGTGCATGCTCTTGTGGTTCTTGCTGCAA GTTTGATGAGGATGACTC TGAGCCAGTTCTCAAGGGTGTCAAATTACATTACACATAA 3' Forward: 5' GGT AGT GAC CTT GAC CGG 3' Tm= 59.8 OA. Reverse: 5' CAA ATT ACA TTA CAC ATA A 3' Tm= 47.4 Forward: 5' ATG TTT ATT TTC TTA TTA TTT 3' Tm= 47.1 C OB. Reverse: 5' TAT GTG TAA TGT AAT TTG ACA CCC 3' Tm= 58.4 Forward: 5' GGT AGT GAC CTT GAC CGG 3' Tm= 59.8 Reverse: 5' GTG TAA TGT AAT TTG ACA CCC TTG 3' Tm: 59.4
Human Anatomy & Physiology (11th Edition)
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ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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Transcribed Image Text:QUESTION 6
To verify the is indeed inside your plasmid, you'd like to do a colony PCR. But you need primers for your reaction. Which of the following primer pairs would
probably work for verifying your insert is actually present in the plasmid?
5' ATGTTTATTTTCTTATTATTTTTTACTCTCACTAGTGGTAGTGACCTTGACCGGTGCACCACTTTTGATG ATGTTCAAGCTCCTAATTACACTCAACATACTTCATCTATGAGGGGGG
TTTACTATCCTGATGAAATTTT
(Very long, but a bunch of nucleotides her
e)....
TCTTGCTTTGTTGCATGACTAGTTGTTGCAGTTGCCTCAAGGGTGCATGCTCTTGTGGTTCTTGCTGCAA GTTTGATGAGGATGACTC
TGAGCCAGTTCTCAAGGGTGTCAAATTACATTACACATAA 3'
Forward: 5' GGT AGT GAC CTT GAC CGG 3' Tm= 59.8
O A.
Reverse: 5' CAA ATT ACA TTA CAC ATA A 3' Tm= 47.4
Forward: 5' ATG TTT ATT TTC TTA TTA TTT 3' Tm= 47.1 C
O B.
Reverse: 5' TAT GTG TAA TGT AAT TTG ACA CCC 3' Tm3 58.4
Forward: 5' GGT AGT GAC CTT GAC CGG 3' Tm3 59.8
OC.
Reverse: 5' GTG TAA TGT AAT TTG ACA CCC TTG 3' Tm: 59.4
Forward: 5' GGT CAC TAC CAC TAG TGA GAG 3' 59.4 C
O D.
Reverse: 5' GTG TAA TGT AAT TTG ACA CCC TTG 3' Tm: 59.4
Forward: 5' GGT AGT GAC CTT GAC CGG 3' Tm= 59.8
OE.
Reverse: 5' GAG CCA GTT CTC AAG GGT G 3' Tm= 60.8
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