During nucleic acid hybridization, the probe is labelledfor DNA stabilityto increase probe-test DNA bindingto identify the location of probe and the test DNA bindingfor amplification You have completed an in vitro mutagenesis experiment to create an G to T mutation in the coding sequenceof the heat shock protein gene. This gene is cloned in a plasmid and you want to confirm that the mutationwas correctly introduced. Which sequencing technology be most appropriate to use practically andeconomically:O MicroarraySanger sequencingRNA sequencingExon sequencing

Answered: Image /qna-images/answer/5907f79c-8e4b-450e-b643-1bb88d8e9526.jpg

Answered: During nucleic acid hybridization, the…

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 1SQ

See similar textbooks

Similar questions

Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…
Why is the annealing temperature different between samples during DNA extraction & marker gene amplification? How is this computed?
What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with the amount of PCRproduct.
The rate of migration of DNA within an agarose gel in the gel electrophoresis technique is primarily based on: O The number of the DNA fragments. The size of the DNA fragments. The concentration of the DNA None
What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with the level of PCRproduct.
describe the two main parts of a plasmid DNA extraction and purification procedure including the purpose of each step
Describe the rationale for column-based DNA extraction and purification.
Cystic fibrosis (CF) test to screen for mutations associated with the CFTR gene. Positive samples will undergo gene sequence analysis. What assay format is used in the screening test.
During which step in the transformation protocol does the plasmid DNA move into the cell? Heat Shock Grow in LB media at 37°C Plate on media with Ampicillin O Incubate the cells on ice with CaCl2 in the absence of plasmid. fg f1o 19 %24 & 5 6. 7 8. 4 5 R Y U P F G J 2 K 3] vの
When you are loading the DNA ladder into your gel the volume needed is 5uL. The appropriate micropipette for this volume is a: 1)p2 2)p200 3)p1000 4)p20
Define the following terms: Genome Bacteriophage λ DNA Plasmid Restriction Enzyme Standard Curve Plasmid Map
is used to seal the phosphodiester backbones between vector and insert. O DNA polymerase O Ligase Restriction enzymes O none of the above

SEE MORE QUESTIONS

Recommended textbooks for you

Biology Today and Tomorrow without Physiology (Mi…

Biology

ISBN:

9781305117396

Author:

Cecie Starr, Christine Evers, Lisa Starr

Publisher:

Cengage Learning