This is a map of PBR322 plasmid Nid If a recombinant plasmid were obtained inserting DNA into the BamHI site, screening for the recombinant plasmid can be done by the following technique. O 1) Plate on nutrient agar plates that contain ampicillin. 2) Plate on nutrient agar plates that contain both ampicillin and tetracycline. O 3) Plate on nutrient agar plates that contain tetracycline, followed by replica plating on ampicillin. 4) Plate on nutrient agar plates that contain tetracycline. 5) Plate on nutrient agar plates that contain ampicillin, followed by replica plating on tetracycline.

Q: The principle of the alkaline method for plasmid DNA isolation is: O A. An acidic solution…

A: Plasmid DNA isolation by alkaline lysis method This method is the most commonly used method to…

Q: Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual).…

A: The lysis buffer contains NaoH and SDS(sodium Dodecyl sulphate) and its used in alkaline lysis of…

Q: A plasmid is cleaved by restriction endonucleases and analyzed by agarose gel electrophoresis.…

A: From the agarose gel image, we get information about the plasmid size and the restriction sites…

Q: 3. A plasmid was cleaved with several restriction enzymes, individually and in combinations. The…

A: Restriction mapping is a technique for mapping an unknown section of DNA by breaking it down into…

Q: The table below shows restriction enzyme pairs used by various teams to digest the YFG and clone it…

A:

Q: Single and double digestion of plasmid pJC716 were performed using the restriction enzymes EcoRI and…

A: Plasmid is a double stranded circular dna molecule that is capable of replicating independently. It…

Q: Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual).…

A: INTRODUCTION E. coli E. coli  are small gram negative motile bacilli which mainly cause urinary…

Q: Draw a representative agarose gel showing the bands, and the direction of current flow as well point…

A: EcoR1 is a restriction enzyme that can cleave a dsDNA at the sequence GTTAAC. They cleave the cite…

Q: The transfection reagent used to introduce plasmids to the HEK293 cells was a lipid reagent. Why is…

A: Transfection is the incorporation of DNA, RNA, or proteins in to the eukaryotic cells for the…

Q: List the major steps that would be required to clone the human insulin gene into a plasmid and to…

A: Gene cloning is the isolation of DNA (deoxyribonucleic acid) sequence or gene of interest and…

Q: Why are the following reagents used?      Neutralizing solution (Plasmid isolation)  Isopropanol…

A: 1) Also, the neutralisation of the arrangement permits the renaturation of DNA. The enormous…

Q: plate your cells on a master plate without any antibiotics.  After colonies appear, you pick and…

A: Recombinant DNA technology is helpful for different gene related experiments.

Q: To verify the is indeed inside your plasmid, you'd like to do a colony PCR. But you need primers for…

A: introduction you can confirm the presence of gene within the vector by two ways either by…

Q: A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A: Introduction :- Recombinant DNA technology ( also known as genetic engineering) involves the use of…

Q: When cloning a foreign DNA fragment into a plasmid, it is often useful to insert the fragment at a…

A: Recombinant DNA technology is the modern technology that is used to engineer DNA molecules that have…

Q: Discuss TWO (2) common methods you could utilize to transform your recombinant plasmids into the…

A: Recombinant plasmids can be transformed into bacterial cells in common molecular biology…

Q: Hey, I need help with this please: Plasmid pRIT450 is 7.0 kb in length and has single PstI, EcoRI,…

A: Given: A Plasmid pRIT450 of  7.0 kb in length. It has single PstI, EcoRI, and BamHI sites. The…

Q: A. Use this data to generate a circular map of the plasmid. Pst I: 10 kb Bam HI: 10 kb Sal I:…

A: Restriction endonucleases are enzymes that cut DNA at specific sites. Restriction endonucleases…

Q: A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic…

A:

Q: plasmid has been modified such that it carries the gene for green fluorescent protein (GFP) under…

A: GFP protein is found in the photoorgans of Aequorea Victoria a jelly fish. It is a 28 kDa protein.…

Q: plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A:

Q: An E. coli Hfr (high frequency recombination) strain is defined as an E. coli strain that Select…

A: Bacteria reproduce via binary fission and splits into two identical daughter cells. Binary fission…

Q: Using the plasmid map of pBCH2.0 provided above, predict how many DNA fragments would be formed if…

A: Restriction enzyme are specific DNA cutters and extremely useful while exploring molecular biology…

Q: You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid…

A: 1bp dsDNA=650 Dalton 1 Dalton=1.66×10^(-24) grams ng is nanograms mcl is micro liters 1 gram=…

Q: State the differences between the plasmid in Figure 1 with pBR322 in terms of their sercening…

A: According to the question, we have to state the differences between the plasmid in figure 1 with…

Q: What role did the transposon play in the transfer of resistance?

A:

Q: plasmid has a total size of 2686 bp. There is an EcoRI recognition site at position 439, BamHI…

A: Plasmid  It is an extra chromosomal piece of DNA in the cell.

Q: Please describe the role of each buffers (P1, P2, N3, PE, and EB) and RNAase A and Lyse blue during…

A: Role of P1 buffer - This buffer usually consists of a basic pH Tris buffer with EDTA…

Q: Jane has isolated a mutant strain of yeast that forms red colonies instead of the normal white when…

A: Plasmids are little pieces of extrachromosomal DNA found in the cytoplasm that can multiply on their…

Q: DNA samples from four individuals were cleaved with the same MW restriction endonuclease. The DNA…

A: Any human biological specimen taken or preserved for the purpose of extracting and analysing DNA in…

Q: The map of plasmid pUC19 is shown below.  The restriction site coordinate is the position of the…

A: Introduction Plasmid Vectors: these are like the artificial vehicle to deliver our gene of insert…

Q: You are studying a new plasmid, and you digest the plasmid with three restriction enzymes: Eco RI…

A: The plasmid map can be created by checking the individual band patterns and band patterns with a mix…

Q: Kha Vu Danels Include: 8DX : Safehy Jor bromie tnto lab repart! Name Section date sheet MAPPING…

A: The restriction enzymes are type of nuclease enzymes. These enzymes have the capability to cleave…

Q: Predict the sizes of the plasmid cleavage products generated on digestion of pBR322 and pGLO with…

A: Plasmids are naturally occurring extra chromosomal DNA molecules and are mostly used for cloning…

Q: Explain the role of the gene that is the selectable marker gene in this experiment.

A: Introduction  In the RDT (recombinant DNA technology), we insert the segment of DNA into the vector…

Q: A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid…

A: The correct answer is E) 1.6 * 1012 cells

Q: You have accidentally torn the labels off two tubes (tube A and tube B), each containing a different…

A: HindIII is a restriction enzyme that is purified from a recombinant E. coli and transfers a plasmid…

Q: The transformation results below were obtained with 10 ul of intact plasmid DNA at nine…

A: Transformation Transformation is the process by which exogenous DNA is transferred into the host…

Q: You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid…

A: Multiple questions asked. I will answer the first question as per guidelines. Please repost the…

Q: You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid…

A:

Q: Why must the restriction enzyme used for the digestion of TOL plasmid be inactivated immediately…

A: Restriction digest of plasmid DNA is done using the naturally occurring restriction enzymes that…

Q: You first need to create a plasmid. What are the minimal components necessary to develop this…

A: Since you have asked many questions, I'm giving you the answer of first 3 Please repost in order to…

Q: The total size of the plasmid is 2686 bp. There is a PstI recognition site at position 439, HindIII…

A: Answer - d. 3 fragments: 447bp, 507bp and 1732bp

Q: Eco R1 Multiple Clone Site (MCS) AmpR Hind 3 LacZ" Plasmid Lacl Amp = Ampicillin Resistance gene Ori…

A: The small DNA piece that is maintained stably in an organism is called a cloning vector. The foreign…

Q: Part A. If student counts 63 colonies on their 10^-6 dilution LB plate. What was the original…

A:

Q: You are a graduate student working to construct a single gene knockout library of Leptospiria…

A: * That constructed a single gene knockout library of Leptospiria kirschneri one the causative agents…

Q: What is a vector? Which vector did you choose? Explain why you made that choice. What enzyme(s) will…

A: Since you have asked a question with multiple subparts, we can only answer the first three subparts…

Q: Describe a technique you could employ to increase the concentration of the TOL plasmid.

A: Molecular biology is the study of biological macromolecules that are involved in the various…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.