(Theme 5) AraC is a transcriptional activator that binds to the AraC binding site and is encoded by the gene araC. lac/ encodes the Lacl repressor that binds to the lac operator sequence. gfp encodes GFP, a fluorescent protein that can be easily visualized. You grow a culture of bacteria where each cell contains all three of the below genetic constructs in their genomes, and add a small amount of the sugar arabinose, which induces the expression of a small amount of AraC protein from a different location in the genome (not shown). Arrows indicate the transcriptional start site. The expression pattern of GFP over time is shown on the right. AraC binding site lac operator arac lacl GFP GFP amount M time A) Briefly explain how GFP expression is turned on after adding arabinose. B) Briefly explain how GFP expression is turned off. C) Briefly explain how GFP is turned on again after it has been turned off.
(Theme 5) AraC is a transcriptional activator that binds to the AraC binding site and is encoded by the gene araC. lac/ encodes the Lacl repressor that binds to the lac operator sequence. gfp encodes GFP, a fluorescent protein that can be easily visualized. You grow a culture of bacteria where each cell contains all three of the below genetic constructs in their genomes, and add a small amount of the sugar arabinose, which induces the expression of a small amount of AraC protein from a different location in the genome (not shown). Arrows indicate the transcriptional start site. The expression pattern of GFP over time is shown on the right. AraC binding site lac operator arac lacl GFP GFP amount M time A) Briefly explain how GFP expression is turned on after adding arabinose. B) Briefly explain how GFP expression is turned off. C) Briefly explain how GFP is turned on again after it has been turned off.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
Please answer parts a, b, and c as shown in the image
Transcribed Image Text:(Theme 5) AraC is a transcriptional activator that binds to the AraC binding
site and is encoded by the gene araC. lacl encodes the Lacl repressor that
binds to the lac operator sequence. gfp encodes GFP, a fluorescent protein
that can be easily visualized.
You grow a culture of bacteria where each cell contains all three of the
below genetic constructs in their genomes, and add a small amount of the
sugar arabinose, which induces the expression of a small amount of AraC
protein from a different location in the genome (not shown). Arrows indicate
the transcriptional start site. The expression pattern of GFP over time is
shown on the right.
AraC binding site lac operator
arac
lacl
GFP
GFP amount
M n
time
A) Briefly explain how GFP expression is turned on after adding arabinose.
B) Briefly explain how GFP expression is turned off.
C) Briefly explain how GFP is turned on again after it has been turned off.
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