The titer of Lauren's phage is 3.7 X 108 pfu/mL. She diluted her phage lysate 1:10 from 10° to 10-7 and use 10μL of lysate for each infection as she followed the procedure for a titer assay.
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- https://youtu.be/w7aIxiZQ60g Multiplexing agglutination https://youtu.be/uWStmyJ5Qc0 This is the multiplexing agglutination. Lab report I don’t really know what to talk about, the data, conclusions and the purpose of this. Need help pleaseYou have conducted serial 10-fold dilutions and measured the cfu (colony forming units) of a Streptococcus pneumoniae culture and also the pfu (plaque forming units) of a phage (virus) that infects the bacteria. You counted 5 cfu in a 0.4 ml sample of a 106 dilution of the bacterial sample. You then counted 50 plaque-forming units (pfu) in a 0.25 ml sample of a 108 diluted sample of the phage culture. What are the cfu/ml of the S. pneumoniae and pfu/ml of the phage cultures before dilution? 5 x 106 cfu/ml and 2 x 109 pfu/ml 4 x 107 CFU/ml and 2 x 109 PFU/ml 1.25 x 108 cfu/ml and 10 x 1010 pfu/ml 1.25 x 107 cfu/ml and 2 x 1010 pfu/mlConsidering counting rules, calculate the initial titre of the sample viral stock if the following plaques were counted in a plaque assay. Show clear sample calculations. Table 1: Plaque assay counts for lambda phage stock titre enumeration of average concentration (PFU/mL) Total dilution of viral stock 1/13500000 1/135000000 1/135000000 plated Volume diluted viral stock 0.1 0.1 0.1 plated (mL) PFU replicate 1 TNTC 275 25 Calculated concentration (PFU/mL) replicate 1 PFU replicate 2 TNTC 239 23 Calculated concentration (PFU/mL) replicate 2 Average concentration (PFU/mL)
- Wil completed a full plate titer assay and there were 25 plaques on his 10^-7 plate. What is the titer of his high titer lysate? 10uL of each dilution were used for the plaque assays. O 2.5X10^11 pfu/mL O 2.5X10^7pfu/mL O 2.5X10^9 pfu/mL O 2.5X10^8 pfu/mL O 2.5X10^10 pfu/mLWrite a lab report on enumeration of lytic viruses : the plaque assay including the sections: introduction, Materials and Methods, Results and Discussion, and citing peer reviewed sources use this data for results section Group Number Number of Plaques Dilution Factor of Plate Original Concentration of Phage 1 58 10^3 5.80E+04 2 37 10^3 3.70E+04 3 44 10^3 4.40E+04 4 63 10^3 6.30E+04 5 36 10^2 3.60E+03 6 65 10^3 6.50E+04 7 260 10^2 2.60E+04 8 39 10^3 3.90E+04 9 178 10^2 1.78E+04 10 192 10^2 1.92E+04 11 274 10^2 2.74E+04 12 224 10^2 2.24E+04 Class Average 3.52E+04If PBR323 is used instead of PUC18, is blue white screening applicable? Justify your answer.
- Why does the antibody titer determination use twofold dilutions ofthe antiserum rather than 10-fold dilutions?You are counting plaques on your plaque assay plates made from serial dilutions of your high titer lysate. Your 10-5 plate has 615 plaques although some are butting up against each other so it is difficult to get an accurate count. Your 10-6 plate has 42 plaques, and your 10-7 plate has only 1 plaque. Which plate would probably yield the most accurate titer calculation of your phage and why is it more trustworthy than the others?The following pictures show the results of a Disk Diffusion Assay for different types of bacteria. For each bacteria, what antibiotic would you recommend be used on the patient? Explain your choice.
- What are the differences between “O” and “H” antigen? Differentiate Widal from Weil-Felix test. The strains of Proteus vulgaris are used in Weil-felix reaction. Explain why.Anita suspects she might have had Covid-19 about four months ago. Currently, she has no symptoms. What tests should be used to find out if that indeed was the case? cell culture Indirect ELISA Direct ELISA real-time qPCROne laboratory method to detect for COVID-19 infection is via real time RT-PCR. The process starts with nasopharyngeal or oropharyngeal swabbing to get a sample. Explain concisely how this technique generally works and how it can be used for testing.