The number of bacterial cells in a culture broth is to be determined by a culturetechnique.Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed withwarm molten agar and poured into a Petri dish. The numbers of bacterial colonyforming units (CFU) after overnight incubation are shown in the table below.What is the number of colony forming units per mL of the culture broth? Choose onlythe most appropriate plate for your calculation. Give your answer as the number only(do not add text for the units). You may use scientific notation with the format1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer awhole number.)Plate DilutionPlate 1 10 dilutionPlate 2 10 dilution-5Plate 3 10 dilutionPlate 4 10 dilutionPlate 5 107 dilution-8Plate 6 100 dilution*Too many to countNumber of colony forming units (CFU)TMTC*TMTC*TMTC*86715418

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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution -3 Plate 1 10 dilution Plate 2 10-4 dilution Plate 3 10-5 dilution Plate 4 10 dilution Plate 5 10 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 1,957 65 21 1 0 m
The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate 1 10 Plate 2 10 Plate 3 10 dilution dilution dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 10 dilution -6 *Too many to count Number of colony forming units (CFU) TMTC* TMTC* 840 28 19 1
The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution Plate 3 107 dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 382 83 10 2 0
You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs. You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution scheme
Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of amL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further dilutedby successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar.After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gramsample of hamburger?
Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________
A sample of water was enumerated using a counting chamber (haemocytometer) and 220 bacterial cells were counted in 25 small squares (each of volume 2.5 x 10-7 cm3). Viable counts were carried out on the same culture using both the pour plate method (incorporating 1 mL samples of a range of dilutions into plate count agar plates) and the Miles & Misra spread plate method, where 0.02 mL samples were spread on sectors of a plate count agar plate. For the pour plate count the average of triplicate samples was 178 colonies per plate of the 10-4dilution. For the Miles and Misra count the average of triplicate samples was 21.3 colonies per sector of the 10-4 dilution. Calculate the total count and the viable pour plate and spread plate counts and suggest possible reasons for the difference between the three different counts.
A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as 1. diagrammed below. Each dilution tube contained 400 ul of diluent and 100 ul was transferred into each tube. TSA plates were inoculated with 100 µul from the last three dilution tubes. a. What is the dilution between each tube shown in the diagram below? Express your answer as a ratio. b. What is the total dilution of tube number 5? Express your answer as a ratio. c. What is the concentration of viable bacteria in the original culture? Express your answer using scientific notation and the units CFU/ml. d. What is the concentration of viable bacteria in tube number 2? Express your answer using the units CFU/ml. e. If you inoculated a TSA plate with 250 µl from tube number 5, how many colonies would you expect to see after the plate was incubated? 1 2 3 5 423 80 13 Number of colonies
Given the illustration and values below, determine the concentration of the original sample. Report results in CFU/mL or colony-forming unit/milliliter. Note that in order to observe the accuracy of results, culture plates with countable colonies between 25-250 CFU are considered in standard bacterial plate count.
A bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows: 1/50, then 10-3 and finally 1/20. If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?
To determine the number of cells in a pure culture of Klebsiella pneumoniae, you have performed a serial dilution using three tubes of sterile saline (9.9 ml each). A sample of 0.1 ml from the culture was added to tube 1. Similarly, 0.1 ml from tube 1 was used to inoculate tube 2, and tube 3 was inoculated using 0.1 ml from tube 2. A nutrient agar plate was then inoculated using 0.1 ml from tube 3 and incubated overnight. The next day, 92 colonies were observed on the plate. How many cfu/ml were in the original culture? Using a Petroff-Hauser counting chamber, the number of cells in the same culture was estimated to be 8.5 • 109 cells/ml. How can you explain these results?
A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as 2. diagrammed below. A volume of 500 µl was transferred into each tube. TSA plates were inoculated with 100 µl from the last three dilution tubes. a. If the dilution between each tube is 102, what is the volume of diluent in each of the 5 dilution tubes? Provide the volume using ml as the units. b. What is the total dilution of tube number 4? Express the total dilution using scientific notation. c. What is the concentration of viable bacteria in the original culture? Express the concentration using scientific notation and CFU/ml as the units. d. If you inoculated a TSA plate with 1.0 ml from dilution tube 4, how many colonies would you expect to form on the plate after incubation? e. If the original culture had a volume of 50ml, what was the total number of viable bacteria in the 50 ml of the original culture? 1 2 3…

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