If you wanted to use the replication of a virus to simulate the process of replication of an animal cell, which type of virus would you choose to simulate the replication process found in an animal cell? OdsDNA -ssRNA dsRNA +ssRNA ssDNA
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- Match each of the following replication classes of viruses with the one description that best describes it. This virus class is composed of segmented and non-segmented helical viruses that carry an RdRp within the virion into the cell. Example: Rabies virus These viruses are non- enveloped, icosahedral, have segmented genomes, and carry their own RNA-dependent RNA polymerase. The viral genome is copied following assembly. Example: Reovirus This is the only class of RNA viruses whose genome is replicated by cellular enzymes and not an RNA-dependent RNA polymerase. Cellular DNA polymerases replicate this virus's non- integrated genome, although transcription is not the initial event that takes place in the process. Example: Parvoviruses In this class of viruses, transcription occurs before genome replication because viral proteins are involved in replicating the genome. Example: Adenovirus This class of viruses forms circular DNA and creates pre- genomic RNA using host RNA polymerases.…Please describe each step mentioned in the figure below illustrating retroviral transfer of DNA into mammalian cells. (Please do not copy and paste from the powerpoint). infecting virus (virion) cel DNA provirus LTR LTR NUCLEUS viral RNA CYTOPLASM core virus particle R- R SSRNA 3b LTR LTR DNAReverse transcriptase would most likely be found in which of the following types of viruses? an RNA-based lytic virus a DNA-based lytic virus an RNA-based lysogenic virus a DNA-based lysogenic virus CO000
- Remdesivir is an antiviral drug used to treat SARS-CoV-2 infections. It is a broad-spectrum antiviral, meaning it has activity against many different viruses. Remdesivir can be incorporated into a new RNA chain as a virus replicates and can interfere with the viral replication machinery. Template RNA Elongation New RNA SARS-CoV-2 RNA polymerase Template groove Nucleotide entrance Nucleotides Remdesivir Figure 1. A representation of remdesivir and the viral replication machinery for SARS-CoV-2. 6. Figure 1 shows that remdesivir "mimics” an important component of RNA replication. Which specific component of RNA replication has a structure like that of remdesivir? 7. Propose a hypothesis about how remdesivir might inhibit the virus's replication process.All viral genomes contain nucleic acid, either DNA or RNA but not both. Viral replication is the formation of biological viruses during the infection process in the target host cell. Viruses must first enter the host cell before viral replication can occur. After gaining entry into a cell, the viral genome has to be first replicated using either the host DNA polymerase or RNA polymerase You are studying two viruses: Virus A is an enveloped RNA virus Virus B is non-enveloped DNA virus Which of the above viruses is likely to have the lowest mutation rate in the host cell? O Virus A because it uses the host RNA polymerase which has lower error rate than DNA polymerase. O Virus A because it uses the host DNA polymerase which has lower error rate than RNA polymerase. O Virus B because it uses the host DNA polymerase which has lower error rate than RNA polymerase. O Virus B because it uses the host RNA polymerase which has lower error rate than DNA polymerase.Which is the usual order of a viral replication cycle when it is making new virions? O Host recognition > replication > entry > uncoating > release O Uncoating > host recognition > replication > entry > release O Prophase > metaphase > anaphase > telophase > cytokinesis Host recognition > entry > uncoating > replication > release MacBook Air DII DD F11 888 F8 F9 F7 F6 F5 F3 F4 & %24 % 7 8 LO
- Which step(s) in the replication cycle of HIV can still occur in the presence of a reverse transcriptase inhibitor? Select all that apply. transcription of viral genes initial viral attachment viral assembly integration of viral DNA into host genome initial viral entryArrange the following list into the correct sequence for part of the cycle of a retrovirus: 1. dsDNA integrated into host DNA 2.viral proteins synthesized on host ribosomes 3. viral DNA uses host enzymes to transcribe viral RNA 4. reverse transcriptase catalyzes synthesis of ssDNA 5. synthesis of second DNA strand (a) 5, 2, 1, 3, 4 (b) 5, 2, 3, 4, 1 (c) 4, 5, 1, 3, 2 (d) 4, 1, 2, 3, 5 (e) 2, 1, 3, 4, 5O O O A new virus is discovered. Its RNA genome encodes several proteins, including a reverse transcriptase enzyme. What can you conclude about replication of this virus? Its replication involves a double-stranded RNA intermediate Its replication involves a DNA copy of its genome Its replication depends entirely on cellular enzymes Its replication is entirely independent of cellular enzymes 00 A deficiency in Base Excision Repair (BER) would primarily sensitize you to mutations caused by radiation, X-rays O Chemicals in smoke, and UV radiation O Unavoidable, spontaneously occurring DNA damage
- OF rasc PDF Teas fill Which of the following is NOT a step in the replication of a retrovirus? A diagram illustrating the process is provided below. PDF UNOFFIC TRANSCR 59°F Mostly cloudy Virus Viral RNA Reverse transcriptase DDDDDD ↓Viral DNA Provirus mRNA mm Proteins mn Viral RNA Protease Plasma membrane Host DNA New virus O The single-stranded DNA forms a double-stranded DNA called a provirus, which is incorporated into the host cell DNA. O After a retrovirus attaches to the host cell, it injects its viral DNA and uses the host cell's machinery and materials to replicate. O After a retrovirus injects its viral RNA into a host cell, it forms a DNA strand by reverse transcription. O When the host cell replicates, a provirus produces the viral RNA needed to produce more virus particles. P O Search hun recc PDF maste omissMatch each stage of the viral replication cycle with the one definition that best describes it. ¡¡¡ The stage at which nascent virions leave the cell The virus adheres to the surface of the cell The viral nucleic acid is released into the cell The stage at which virions become infectious The construction of new virions composed of viral proteins and the replicated genome The stage at which the viral genome is copied The virus passes into the cell from the extracellular environment 1. Attachment 2. Replication 3. Maturation 4. Release 5. Penetration 6. Uncoating 7. AssemblyYour friend hasn't been feeling well, and you think that she should get tested because it could be a virus or even multiple viruses! Nancy is conducting a qPCR experiment to detect how much of virus A, B, and/or C your friend is infected with. All three viruses contain RNA as their genetic material. Nancy takes a saliva sample and is excited to begin. However, it's her very first time conducting qPCR so a few mishaps occurred during this experiment. Unfortunately... ● Nancy forgets to reverse transcribe the RNA of virus A, and • The cDNA of virus C contains a mutation where the probe is designed to bind, and • Nancy uses the same color fluorescent dye for viruses B and C Fortunately, all the probes consisted of a fluorescent and quenching dye. Which qPCR graph is most representative of Nancy's experiment after several cycles of qPCR? Explain why the answer you chose is correct. A. (₁ Fluorescence B B # of Cycles A A # of Cycles с B. D. Fluorescence B # of Cycles B # of Cycles с