Q: Explain the reasons you would use a negative stain?
A: In a negative stain the slide is actually stained. This is because the negatively charged stain…
Q: (list color and example for Gram + and Gram -) Acid Fast (list color and example for Acid Fast + and…
A: To get the remaining sub-parts solved, please repost the complete question and mention the sub-parts…
Q: What is the importance of Gram-staining?
A: Introduction : Gram Staining, Commonly Known As Gram's Method, Is A Staining Technique That Is Used…
Q: Explain the significance of crystal violet, iodine, Grams decolorizer, and safrin make in Gram…
A: Gram staining is a common laboratory technique in microbiology that is used to differentiate…
Q: Which stain is most frequently used to distinguish differences between thecell walls of medically…
A: The cell walls of bacteria are made up of peptidoglycan- a layer of polysaccharide chains cross…
Q: what color would you expect for a gram positive microorganism for each incorrect mistakes while…
A: "Gram staining" is a technique used in microbiology. It is a method in which a bacterium can be…
Q: The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with…
A: Mycobacteria are immobile, slow-growing (doubling time = 24 h), rod-shaped, gram-positive bacteria…
Q: If you have a mixed (Gram positive and Gram negative) sample, and you complete a Gram stain, and all…
A: On the basis of lipid content in cell wall of bacteria these are classified into two types : Gram…
Q: Give two reasons for the differential staining between gram + and gram - organisms
A: Differential Staining is all those staining methods that employ more than one stain to distinguish…
Q: In gram staining, could colors other than red be used as a counterstain?
A: A counterstain is a stain with color contrasting to the principle stain, making the stained…
Q: In your own words, explain how gram staining is done and how it works.
A: Gram staining is a popular method for distinguishing between two large groups of bacteria based on…
Q: Which of the following places the steps of the Gram stain in the correct order? 1-Alcohol-acetone…
A: There are two types of bacteria, i.e gram-positive and gram-negative.
Q: Give the principle of acid fast staining and gram staining.
A: Microorganisms are extremely small in size and hence cannot be visualized directly. Specialized…
Q: What is the importance of Gram-staining
A: Gram staining was created by Christian gram Bacteria with Peptidoglycan in their cell walls are…
Q: -What are the steps of gram staining -Which of the four steps is the most important and why? - What…
A: Based on the chemical and physical characteristics of their cell walls, the Gram stain is an…
Q: Why is it important to perform a gram stain for any type of bacteria? After performing the gram…
A: The gram staining is a procedure that is useful in classifying the bacteria based on retention of…
Q: The Gram Stain is able to stain bacterial cells differently depending upon the structure of their…
A: Bacterial cell wall is made up of peptidoglycan. it prevents the cell from bursting when it absorbs…
Q: What would be the result of the gram stain if acid alcohol is used as a decolorizer rather than the…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…
A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…
Q: list 4 advantages and 4 limitations of the Gram staining process
A: Gram staining process is the procedure which is uses differential gram stain which has two dyes to…
Q: The counterstain in the Gram stain is: A basic dye A negative stain A mordant An acid dye Necessary…
A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Histology, as well…
Q: Why is mordant necessary for the staining of bacterial flagella? Answer in not more than 3…
A: In contrary to negative staining, which leaves the bacteria unstained against a dark backdrop,…
Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: List the steps in a Gram stain.
A: Introduction: The principle of Gram's stain depends upon the Gram's reaction. The Gram's reaction…
Q: the purpose of a mordant in Gram stain is a. to prevent the crystal violet from leaving the cell…
A: A mordant or color fixative is defined as a substance used to set (for example tie) colors on…
Q: What is the purpose of iodine in the Gram stain? To act as a counterstain None of the other…
A: There are various staining techniques that are used to stain different cellular structures. The Gram…
Q: List the Comparison of Positive and Negative Stains.
A: A light microscope is the most commonly used microscope for viewing specimens. The specimens that…
Q: Define the following terms: a.Simple staining b.Complex medium c.Defined (synthetic) medium
A: Staining is used to visualise the bacteria i clinical specimen under a light microscope. Bacterias…
Q: Explain the different results in the Gram stain are due to differences in the structure of the cell…
A: There are many types of bacteria and bacterial species. They are prokaryotic cell which has a cell…
Q: Using the acid-fast stain's name as a guide, what would be a logical alternative name for the Gram-…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: The Gram staining procedure involves multiple steps with use different reagents. Complete the table…
A: Step 1 Christian Gram in 1884 developed a technique of staining bacteria called Gram staining.…
Q: Discuss the various theories on the Gram stain.
A: Bacteria can be classified into two major types according to gram staining: Gram positive. Gram…
Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: please label this in the correct order for gram staining. crystal violet, iodine, alcohol, and…
A: Gram staining is a method of staining useful for identifying and classifying bacteria into two major…
Q: Select all of the following that represent examples of positive stains. Check All That Apply…
A: Staining is the common laboratory procedure which can be specific and general according to the use.…
Q: Write out the steps for this specfic stain. List them in the correct order
A: Organisms can be tentatively recognized via developing them on media and searching at morphology or…
Q: The acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C.…
A: Acid fast bacteria are gram positive bacteria . They are unique among prokaryotes and normal gram…
Q: List the primary stain, secondary stain, mordant, and decolorizer for the gram stair and write them…
A: The strains in the plate is give on two strains the primary strain and secondary strain. The primary…
Q: You use gram staining procedure to stain a bacterium that lacks a cell wall. What color will the…
A: Gram staining is a special technique in which bacteria are stained with gram stain. It is used to…
Q: Explain how you would troubleshoot a Gram stain of a known G(-) organism that stained purple.
A: Gram staining is used to differentiate bacteria based on their cell wall constituents. Gram-positive…
Q: Which of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine C.…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: Gram negative bacteria do not retain the stain when washed with alcohol.Comment.
A: Gram staining is a most important technique in the microbiology to differentiate the group of…
Q: What is the purpose of iodine in the gram staining process? Explain.
A: Gram stains are used to distinguish between bacteria with and without cell walls. When the gramme…
Q: employ a chemical (using a reagent, not a physical method) mordant? Gram stain; Acid-fast stain.…
A: The main function of modern in staining procedure is to retain the original stain or dye. It is a…
Q: You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look…
A: The gram-positive and gram-negative bacteria can be differentiated based on their cell wall elements…
Q: Imagine you accidentally applied water during the decolorization step instead of ethyl alcohol.…
A: Gram positive bacteria has thick peptidoglycan layer and thin outer membrane. Gram negative bacteria…
Q: The bacterium Bacillus anthracis was stained with gram staining, endospore staining, acid-stain…
A: Bacillus anthracis is a Gram-positive and rod-shaped bacterium that causes anthrax, a deadly disease…
Q: start with the results of your Gram Stain. In the spaces labeled appearance& observations, indicate…
A: Gram stain : It is a test that checks for the bacteria at the site of suspected infection such as…
Q: What is the identification of the unknown bacteria
A: Tetrad form are only seen in Gram positive cocci bacterium.
Q: List the steps of the gram staining procedure in proper sequence and indicate the color of…
A: Gram staining is a method of staining used to distinguish and classify bacterial species into two…
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- What is Ziehl Neelsen bacterial staining technique, and what is the target organisms and the purpose of its application? LONG ANSWER NEEDED, DETAILED. Thank you i’ll rate.please label this in the correct order for gram staining. crystal violet, iodine, alcohol, and safraninThe following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope at 10x magnification. Samples were stained with Lugols solution for better viewing. Differentiate these two image
- The first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stainWhat are the principle and basic concepts of Simple staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)List the primary stain, secondary stain, mordant, and decolorizer for the gram stain, and write them in the order that you would use them. Make sure to label which is which.
- Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide? Paragraph BIUExplain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin
- The bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?The acid-fast stain is used to stain _____. The decolorizer used to destain the cells is ______. Positive cells that retain the primary stain are ______, while negative cells are counterstained with _____ and will have a ______ color. *Please use answers from the options provided in the image below.Application of differential staining technique.