TESTING THE HYPOTHESIS FIGURE 5.6 Evidence that adult female mammals contain one X chromosome that has been permanently inactivated. (photo): O Lutz Slomianka Starting material: Several small skin samples taken from a woman who was heterozygous for the fast and slow alleles of G-6-PD. Experimental level Conceptual level 1.Mince the tissue to separate the individual cells. 250 im Mince and grow. X chromosome Mince. with slow -Barr body allele active 2.Grow the cells in a liquid growth medium and then plate (sparsely) onto solid growth medium. Each cell divides to form a clone of many cells. Liquid growth medium with cells -X chromosome with fast allele active Plate sparsely. Solid medium with cells Individual cell Grow cells several days. Clone of cells 3. Take nine isolated clones and grow in liquid cultures. (Only three are shown here.) Grow each clone in a Many cell divisions separate flask. Produces a clone of cells 4.Take cells from the liquid cultures, lyse cells to obtain proteins, and subject to gel electrophoresis. (This technique is described in the Appendix.) Sample Control Origin Note: As a control, lyse cells from step 1, and subject the proteins to gel electrophoresis. The control is derived from several small skin samples from a heterozygous woman. Slow Fast From a clone with From clones with the fast allele active the slow allele active

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Let’s suppose an X-linked gene in mice exists as two alleles, which
we will call B and b. X-chromosome inactivation, a process in
which one X chromosome is turned off, occurs in the somatic cells
of female mammals . Allele B encodes an mRNA that is 900 nucleotides long, whereas allele b contains a small deletion that shortens the mRNA to a length of 825 nucleotides.
Draw the expected Northern blot that will be obtained using
mRNA isolated from somatic tissue of the following
mice:
Lane 1. mRNA from an XbY male mouse
Lane 2. mRNA from an XbXb female mouse
Lane 3. mRNA from an XBXb female mouse.
Note: The sample taken from the female mouse is not from a
clone of cells. It is from a tissue sample, like the one shown.

TESTING THE HYPOTHESIS
FIGURE 5.6 Evidence that adult female mammals contain one X chromosome that has
been permanently inactivated.
(photo): O Lutz Slomianka
Starting material: Several small skin samples taken from a woman who was heterozygous for the fast and slow alleles of G-6-PD.
Experimental level
Conceptual level
1.Mince the tissue to separate the
individual cells.
250 im
Mince and grow.
X chromosome
Mince.
with slow
-Barr body
allele active
2.Grow the cells in a liquid growth
medium and then plate (sparsely)
onto solid growth medium. Each cell
divides to form a clone of many cells.
Liquid
growth
medium
with cells
-X chromosome with
fast allele active
Plate sparsely.
Solid medium
with cells
Individual cell
Grow cells
several days.
Transcribed Image Text:TESTING THE HYPOTHESIS FIGURE 5.6 Evidence that adult female mammals contain one X chromosome that has been permanently inactivated. (photo): O Lutz Slomianka Starting material: Several small skin samples taken from a woman who was heterozygous for the fast and slow alleles of G-6-PD. Experimental level Conceptual level 1.Mince the tissue to separate the individual cells. 250 im Mince and grow. X chromosome Mince. with slow -Barr body allele active 2.Grow the cells in a liquid growth medium and then plate (sparsely) onto solid growth medium. Each cell divides to form a clone of many cells. Liquid growth medium with cells -X chromosome with fast allele active Plate sparsely. Solid medium with cells Individual cell Grow cells several days.
Clone of cells
3. Take nine isolated clones and grow in
liquid cultures. (Only three are shown
here.)
Grow each
clone in a
Many cell divisions
separate
flask.
Produces a clone of cells
4.Take cells from the liquid cultures, lyse
cells to obtain proteins, and subject to
gel electrophoresis. (This technique is
described in the Appendix.)
Sample
Control
Origin
Note: As a control, lyse cells from
step 1, and subject the proteins to gel
electrophoresis. The control is derived
from several small skin samples from a
heterozygous woman.
Slow
Fast
From a clone with From clones with
the fast allele active the slow allele active
Transcribed Image Text:Clone of cells 3. Take nine isolated clones and grow in liquid cultures. (Only three are shown here.) Grow each clone in a Many cell divisions separate flask. Produces a clone of cells 4.Take cells from the liquid cultures, lyse cells to obtain proteins, and subject to gel electrophoresis. (This technique is described in the Appendix.) Sample Control Origin Note: As a control, lyse cells from step 1, and subject the proteins to gel electrophoresis. The control is derived from several small skin samples from a heterozygous woman. Slow Fast From a clone with From clones with the fast allele active the slow allele active
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