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Table 1: Microscope Magnification Magnifying power Objective 4X Objective Eyepiece Total name Scanner Low power 100 X High power 40 X PREI

Table 1: Microscope Magnification Magnifying power Objective 4X Objective Eyepiece Total name Scanner Low power 100 X High power 40 X PREI

Biomedical Instrumentation Systems
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter15: Instrumentation In Medical Imaging
Section: Chapter Questions
Problem 9P
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PRELAB: Calculating Magnification Each lens makes the object look larger by a specified amount. To determine the total magnification, multiply the magnification of the eyepiece and the objective being used. Table 1: Microscope Magnification Magnifying power Objective Objective Eyepiece Total name Scanner 4 X Low power 100 X High power 40 χ PRELAB: Estimating the Size of an Object using a Microscope You will not be able to use a ruler to directly measure the size of a cell. Instead, you will use an indirect method by comparing the cell to the size of something you already know. The diameter of the microscope's field of view as seen through the eyepiece is a convenient length to use. A very small unit of measurement, the micron (µm) is used for measuring microscopic objects. The conversions are: 1 um = 0.001 mm 1 mm = 1000 µm Table 2: Field of View Diameters Objective Diameter name millimeter (mm) micron (um) Scanner 4 Low power High power 0.5 To estimate the size of an object, divide the diameter by the approximate number of specimens that could fit across that diameter. For example, if you estimate that 20 cells could fit across the diameter when viewing under low power, then the size is: diameter amt of objects 2000 un 20 cells 100 Hn/cell %3D
Transcribed Image Text:PRELAB: Calculating Magnification Each lens makes the object look larger by a specified amount. To determine the total magnification, multiply the magnification of the eyepiece and the objective being used. Table 1: Microscope Magnification Magnifying power Objective Objective Eyepiece Total name Scanner 4 X Low power 100 X High power 40 χ PRELAB: Estimating the Size of an Object using a Microscope You will not be able to use a ruler to directly measure the size of a cell. Instead, you will use an indirect method by comparing the cell to the size of something you already know. The diameter of the microscope's field of view as seen through the eyepiece is a convenient length to use. A very small unit of measurement, the micron (µm) is used for measuring microscopic objects. The conversions are: 1 um = 0.001 mm 1 mm = 1000 µm Table 2: Field of View Diameters Objective Diameter name millimeter (mm) micron (um) Scanner 4 Low power High power 0.5 To estimate the size of an object, divide the diameter by the approximate number of specimens that could fit across that diameter. For example, if you estimate that 20 cells could fit across the diameter when viewing under low power, then the size is: diameter amt of objects 2000 un 20 cells 100 Hn/cell %3D
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