Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length | (mm) Working distance (mm) Linear magnification (X)| Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)
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- What information can be obtained from the slides prepared by wet mount and hanging drop technique for microscopic observation? Give at least three (3) information. What are the limitations of each method. (Provide 2 limitations)Discussion: 1) What does calibrating the microscope mean? Why do we calibrate the microscope? 2) What is the ocular calibration scale for each objective? Why is important to calibrate each objective?Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.
- Write T (True) or F (False) for the following statements. 1. The objective lens is the one nearest to the observer's eye. 2. Only special lens paper should be used to clean the objectives, oculars, and the condenser of the microscope. 3. The working distance is the distance between the specimen and the objective lens. _4. Magnifying a blurred image generally reveals further details. 5. When a microscope is parfocal, you should not have to use the coarse adjustment at higher magnification. _6. The total magnification of the object seen at high dry power is approximately 40X. 7. Immersion oil can be used to increase resolution of all the objective lenses in a brightfield microscope. _8. Always be sure to oil your microscope lenses before returning the instrument to its designated space. 9. Animals will not survive without mitochondria because they will not be able to make ATP. 10. The use of normal saline solution (NSS) in viewing cheek cells is to prevent lysis or crenation of cells.Which of the following statements are true in the context of the microscope's field of view? I. It is the observable area within a circular space as defined by the lenses II. FOV is the smallest on the lowest power objective III. FOV is the maximum area visible through the microscope eyepiece IV. When you switch to a higher power, the field of zooms out towards the centerCompare and contrast the operation of optical microscopy and TEM in terms of Contrast (explain all different types for each microscopy) Note: Wherever possible, explain what factors affect or control each features.
- Previous question: For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope. - The Answer I got for this question was 3/4mm.Answer the following questions: Why are most cells so small? Why cell size is limited? List two instances when the coarse adjustment knob is never used Why is immersion oil used with the 100X objective? List two common problems associated with using the microscope and how you would go about solving it. When should the lenses be cleaned? What is the correct way to clean them?a. How was the specimen prepared for the microscopy technique applied? (for e.g. stained with H&E stain, Gram stain, unstained) b. What is the microscopy technique and magnification used to obtain this image? c. What is the basic principle of image formation using this microscopy technique? d. What can be observed and concluded from the image of the specimen? e. Are there any potential aberrations present in this specimen image? Describe these and how they may affect interpretation of the result.
- Calculate below the total magnification for each ocular/objective combination on your microscope. Ocular Objective Total Magnification 1. What is the magnification of the oculars on your microscope? 3. What are the magnifications of the objectives on your microscope?Label the parts of the microscope on the diagram below: arm, stage, base, ocular, revolving nosepiece, objective (4x, 10x, 40x), course focus, fine focus, condenser & iris diaphragm (these are marked by the same line)Which of the following microscopy techniques can be used in live cell imaging in a live animal. 1. Structured illumination microscopy 2. Stimulated emission depletion microscopy 3. Photoactivated localization microscopy 4. Two-photon excitation microscopy 5. Total internal reflection microscopy 6. Light-sheet microscopy O4 and 6 O2, 3 and 6 O1 and 4 3 and 5 O2 and 4