Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures you have learned about during the semester. You must write up your OWN dichotomous key for all the possible unknown organisms listed on above. Writing this key requires you use the same type of reasoning used in the Dichotomous key lab. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. DO NOT simply copy the dichotomous key from you lab manual or from other sources (e.g. Bergey’s manual). The organisms are not the same as the ones used in this exercise. Also, because of genetic variability between organisms, the results may not be same as in our lab book or in other sources. Use the tables in your lab notes to create your key. The key must include a source for each test. You can use footnotes in your key or just include the information right next to test name. Since the tests we use come from either the lab manual or the Lab Notes, reference each test by citing the exercise number or the Lab Notes. Example: "6.5% NaCl Broth, Exam 3 Lab Notes page 6" or "Nitrate Reduction Test, Ex. 5-7". 4. Your dichotomous key must be typed. If you need more room than one page, feel free to use two. You can use Powerpoint, Word, or the Google equivalents to type up your key. Use text boxes and connectors to make a flow chart. Also, there is a cool site called Creately (Links to an external site.) that allows you to make dichotomous keys. Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures you have learned about during the semester.

  1. You must write up your OWN dichotomous key for all the possible unknown organisms listed on above. Writing this key requires you use the same type of reasoning used in the Dichotomous key lab. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. DO NOT simply copy the dichotomous key from you lab manual or from other sources (e.g. Bergey’s manual). The organisms are not the same as the ones used in this exercise. Also, because of genetic variability between organisms, the results may not be same as in our lab book or in other sources. Use the tables in your lab notes to create your key.
  2. The key must include a source for each test. You can use footnotes in your key or just include the information right next to test name. Since the tests we use come from either the lab manual or the Lab Notes, reference each test by citing the exercise number or the Lab Notes. Example: "6.5% NaCl Broth, Exam 3 Lab Notes page 6" or "Nitrate Reduction Test, Ex. 5-7". 4.
  3. Your dichotomous key must be typed. If you need more room than one page, feel free to use two. You can use Powerpoint, Word, or the Google equivalents to type up your key. Use text boxes and connectors to make a flow chart. Also, there is a cool site called Creately (Links to an external site.) that allows you to make dichotomous keys.

 

 

Possible Organisms

  • Alcaligenes faecalis
  • Enterobacter aerogenes
  • Enterococcus faecalis
  • Escherichia coli
  • Proteus vulgaris
  • Pseudomonas aeruginosa
  • Salmonella arizoniae
  • Staphylococcus aureus
  • Staphylococcus epidermidis
  • Staphylococcus saprophyticus
  • Streptococcusbovis
  • Streptococcus pyogenes

 

 

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