list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet endospore staining methods. (omit the rinsing steps)

Q: The first step in identifying an organism from a pure working culture is to perform a(n) __________…

A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…

Q: Explain why only gram-negative cells undergo decolorization during the gram staining procedure.

A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…

Q: What are the sources of error in performing Gram staining?

A: Bacteria can be divided into two categories namely gram positive(with thin layer of peptidoglycan)…

Q: Define fluorescent staining.

A: The advent of microscopy started with the development of optical microscopy. Then the level of…

Q: You receive a bacterial culture in the lab and your job is to figure out information which will be…

A: Best option to stain a bacterial culture is Gram Stain Method. It is used to determine gram status…

Q: Why must heat or a surface-active agent be used with application of the primary stain during…

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: Please discuss why staining is important in visualizing microbes under the microscope. What…

A: Staining is an essential step in visualising microbes under the microscope. THE NECESSITY OF…

Q: What are the principle and basic concepts of DIFFERENTIAL staining?

A: The simple dye is used in the technique of simple staining to highlight only the specific structures…

Q: Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed…

A: Bacteria have cell walls made up of polysaccharides that give them strength.Bacterial cell walls…

Q: Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain…

A: In capsule staining procedure “we do not heat fix and rinse the smear with water” as heat and water…

Q: I need to discribe the "the colors of gram+ and gram (-) bacteria" in each independent step of the…

A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…

Q: Which step is the most crucial or most likely to cause poor results in the Gram stain?

A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…

Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…

A: Gram staining may be a staining strategy utilized to distinguish and recognize bacterial living…

Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…

A: Simple staining: Simple stain uses only one stain for staining bacterial cells. It gives basic…

Q: Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methods

A: Ziehl neelsen staining principle The presence of mycolic acids in the cell walls of acid-fast…

Q: What is the principle of Giemsa staining and  components of Giemsa stain?

A: The cells are the basic structural and fundamental unit of the living system. It consists of…

Q: What are the principle and basic concepts of NEGATIVE staining?

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: Name all the reagents used in Gram-staining and their roles in the staining procedure.

A: Staining is a strategy used to improve contrast in examples, for the most part at the minuscule…

Q: Describe one error in the staining procedure that would result in a Gram-positive bacterium…

A: Gram staining is a differential staining technique that differentiates between a gram-positive and a…

Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct)   a…

A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…

Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…

A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…

Q: What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method?

A: Kinyoun staining is a technique used to stain bacteria. Specifically, acid-fast species of the…

Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used

A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…

Q: Types of Differential Staining techniques.  1. Gram stain 2. Acid-fast stain     Please…

A: Staining is used in microscopy to highlight the structures to be viewed under microscope. It is of…

Q: Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative staining

A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…

Q: Briefly discuss the difference between simple and differential staining? Which is better suited for…

A: Staining is a laboratory technique in which various coloured dyes are used to highlight the specimen…

Q: What are the principle and basic concepts of Simple staining

A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…

Q: Give three situations where the negative staining procedure would be used.

A: Negative staining is a specimen preparation technique that requires an acidic dye such as India Ink…

Q: Explain the principle of Gram staining. (You do not need to describe the procedure step by step).

A: The danish Bacteriologist Christian gram discovered this differential technique in order to…

Q: About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this…

A: Gram staining technique is a differential test based on the composition of bacteria cell wall. It…

Q: Please provide the principle of gram staining method.

A: Introduction: Gram staining is the most widely used differential staining. It is based on the…

Q: What is the difference between Giemsa and Wright stain?

A: Staining is a technique used to enhance contrast in samples, generally at the microscopic level.…

Q: Why must young cultures be used when doing a Gram stain?

A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…

Q: What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain,…

A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…

Q: The acid fast technique is also known as:  A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C.…

A: Acid fast bacteria are gram positive bacteria . They are unique among prokaryotes and normal gram…

Q: Which step in the Gram stain procedure is most prone to error? If done incorrectly, how might that…

A: The differential stain is used to distinguish between different structures and cellular components.…

Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…

A: The staining is done to enhance the visualization of cell and certain cellular component under a…

Q: The primary stain used in the endospore stain is:    A. Carbolfuchsin Stain B. Methylene  Blue C.…

A: To differentiate between the vegetative cells and the endospores, a differential staining technique…

Q: List the step-by-step procedure of Gram staining.

A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…

Q: How does the age of a culture affect the Gram stain reaction? What is an optimum culture age for a…

A: To identify the unknown bacteria this gram staining is used. This staining technique differentiate…

Q: Which of the following is/are true regarding the acid-fast stain? (There may be more than one…

A: Answer  First option  Non-acid-fast microbes appear blue in a completed acid-fast stain.

Q: Why is it incorrect to say that the GFP is a “staining” method?

A: Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that…

Q: What macromolecules does DAPI stain? What are its advantages and disadvantages?

A: Genetics is the branch of biology that deals with genetic material like DNA, RNA, inheritance.…

Q: why is gram staining important

A: Gram staining is a laboratory technique which is used to differentiate between different types of…

Q: what would be the medical significance of finding a positive endospore stain result from bacilli…

A: Endospores are thick walled structures found in the genus Bacillus and Clostridium.

Q: The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in…

A: Acid fast bacteria are those which responds to acid fast staining . Most of the members of…

Q: Briefly, in your own words, describe the steps of the gram staining procedure.

A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…

Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…

A: Need to explain the primary stain in the Kinyoun acid-fast stain.

Q: What are the principle and basic concepts of GRAM STAIN?

A: Gram staining is among the most important microbiology staining procedures. It was first invented in…

Q: In preparing bacterial smear, why do we need to pass the slide over the flame?

A: since this is a multi-part question, so I am answering only 1st part. please upload other questions…

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.